The SFTSV Nonstructural Proteins Induce Autophagy to Promote Viral Replication via Interaction with Vimentin

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly identified phlebovirus associated with severe hemorrhagic fever in humans. Studies have shown that SFTSV nucleoprotein (N) induces BECN1-dependent Autophagy to promote viral assembly and release. However, the function of other SFTSV proteins in regulating Autophagy has not been reported. In this study, we identify SFTSV NSs, a nonstructural protein that forms viroplasm-like structures in the cytoplasm of infected cells as the virus component mediating SFTSV-induced Autophagy. We found that SFTSV NSs-induced Autophagy was inclusion body independent, and most phenuivirus NSs had autophagy-inducing effects. Unlike N protein-induced Autophagy, SFTSV NSs was key in regulating Autophagy by interacting with the host's vimentin in an inclusion body-independent manner. NSs interacted with vimentin and induced vimentin degradation through the K48-linked ubiquitin-proteasome pathway. This negatively regulating Beclin1-vimentin complex formed and promoted Autophagy. Furthermore, we identified the NSs-binding domain of vimentin and found that overexpression of wild-type vimentin antagonized the induced effect of NSs on Autophagy and inhibited viral replication, suggesting that vimentin is a potential Antiviral target. The present study shows a novel mechanism through which SFTSV nonstructural protein activates Autophagy, which provides new insights into the role of NSs in SFTSV Infection and pathogenesis. IMPORTANCE Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly emerging tick-borne pathogen that causes multifunctional organ failure and even death in humans. As a housekeeping mechanism for cells to maintain steady state, Autophagy plays a dual role in viral Infection and the host's immune response. However, the relationship between SFTSV Infection and Autophagy has not been described in detail yet. Here, we demonstrated that SFTSV Infection induced complete autophagic flux and facilitated viral proliferation. We also identified a key mechanism underlying NSs-induced Autophagy, in which NSs interacted with vimentin to inhibit the formation of the Beclin1-vimentin complex and induced vimentin degradation through K48-linked ubiquitination modification. These findings may help us understand the new functions and mechanisms of NSs and may aid in the identification of new Antiviral targets.

Beclin1-vimentin complex; IBs; NSs; SFTSV; autophagy.