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  3. In vitro and in vivo investigations on arsenic-induced cartilage degeneration in osteoarthritis

In vitro and in vivo investigations on arsenic-induced cartilage degeneration in osteoarthritis

  • J Hazard Mater. 2023 Sep 21;461:132570. doi: 10.1016/j.jhazmat.2023.132570.
Godagama Gamaarachchige Dinesh Suminda 1,  Yunhui Min 1,  Min Woo Ha 2,  Mrinmoy Ghosh 3,  Dong-Sun Lee 4,  Young-Ok Son 5
Affiliations

Affiliations

  • 1 Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju-si 63243, Republic of Korea.
  • 2 Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju-si 63243, Republic of Korea; Jeju Research Institute of Pharmaceutical Sciences, College of Pharmacy, Jeju National University, Jeju-si 63243, Republic of Korea.
  • 3 Department of Animal Biotechnology, Faculty of Biotechnology, College of Applied Life Sciences Jeju National University, Jeju-si 63243, Republic of Korea; Department of Biotechnology, School of Bio, Chemical and Processing Engineering (SBCE), Kalasalin-gam Academy of Research and Educational, Krishnankoil 626126, India.
  • 4 Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju-si 63243, Republic of Korea; Health Materials Core-Facility Center, Jeju National University, Jeju-si 63243, Republic of Korea; Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, Republic of Korea; Practical Translational Research Center, Jeju National University, Jeju 63243, Republic of Korea. Electronic address: dongsunlee@jejunu.ac.kr.
  • 5 Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju-si 63243, Republic of Korea; Department of Animal Biotechnology, Faculty of Biotechnology, College of Applied Life Sciences Jeju National University, Jeju-si 63243, Republic of Korea; Health Materials Core-Facility Center, Jeju National University, Jeju-si 63243, Republic of Korea; Practical Translational Research Center, Jeju National University, Jeju 63243, Republic of Korea. Electronic address: sounagi@jejunu.ac.kr.
PMID: 37742380 DOI: 10.1016/j.jhazmat.2023.132570
Abstract

Heavy metals found in the environment, including arsenic (As) pose significant risks to human health and present a risk factor for osteoarthritis (OA). This study researched the impact of As on cartilage degeneration by focusing on the role of As in causing OA in mice. We employed chemical inhibition and inductively coupled plasma mass spectrometry analyses to identify the effect of As on chondrocytes as well as studying its accumulation in organs after oral administration in mice. Additionally, the study examined the effect of intra-articular As treatment on the levels of crucial catabolic factors, namely Hif-2α (Epas1) and Zip8 (Slc39a8), during OA progression. Mice that were administered As orally in conjunction with surgically induced joint instability, had heightened cartilage destruction compared to wild-type mice. Quantitative analysis revealed a significant increase in Hif-2α and Zip8 mRNA expression (p = 0.0352,0.0004 respectively) and protein expression (p = 0.0101,0.008 respectively) post oral administration. Our findings illustrated the role of As in influencing crucial cellular functions that are triggered by Reactive Oxygen Species. These events consequently activate the Akt/Hif-2α/NF-κB pathways, leading to disruptions in articular cartilage homeostasis. This study provides a comprehensive understanding of the impact of As on the development of osteoarthritis.

Keywords

Arsenic; Cartilage degeneration; Osteoarthritis; Reactive oxygen species.

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