2. NF-κB Cell Cycle/DNA Damage Autophagy Apoptosis
  3. IKK Deubiquitinase Autophagy Apoptosis NF-κB
  4. BAY 11-7082

BAY 11-7082  (Synonyms: BAY 11-7821)

目录号: HY-13453 纯度: 99.93%
COA 产品使用指南

摩尔计算器

BAY 11-7082 (BAY 11-7821) 是一种 IκBα 磷酸化和 NF-κB 抑制剂，选择性且不可逆地抑制 TNF-α 诱导的 IκB-α 磷酸化，并减少 NF-κB 和粘附分子的表达。BAY 11-7082 抑制泛素特异性蛋白酶 USP7 和 USP21 (IC50分别为 0.19, 0.96 μM)。BAY 11-7082 抑制脂质体中的 gasdermin D (GSDMD) 孔形成以及炎性体介导的细胞凋亡和人和小鼠细胞中IL-1β的分泌。

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BAY 11-7082 Chemical Structure

BAY 11-7082 Chemical Structure

CAS No. : 19542-67-7

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥550
In-stock
5 mg ￥500
In-stock
10 mg ￥650
In-stock
50 mg ￥2200
In-stock
100 mg ￥3000
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

BAY 11-7082 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 306 篇科研文献

WB
RT-PCR

    BAY 11-7082 purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Mar;120(3):3898-3910.  [Abstract]

    Cells pretreated with SP600125 show a decreased proapoptotic Bax expression and increase antiapoptotic Bcl-2 formation when JNK pathway is blocked. Inhibition of p38 by SB202190 significantly enhanced Bcl-2 expression. Pretreatment with BAY 11-7082 to inhibit NF‐κB markedly enhance Baxm and decrease Bcl-2 expression compared with the ACR-treated group.

    BAY 11-7082 purchased from MCE. Usage Cited in: BMC Cancer. 2019 Jan 8;19(1):30.  [Abstract]

    The representative images and analysis of western blot for IκBα、p-IκBα and nuclear p65 in CRC cells and expression levels of E-cadherin, N-cadherin and fibronectin in SW620 and HCT116 cells are measured by western blot treated with BAY 11-7082 (inhibitor of NF-κB pathway).

    BAY 11-7082 purchased from MCE. Usage Cited in: Evid-Based Compl ALlt. 2019 Jan 13;2019:9406342.  [Abstract]

    The expressions of p-p65, COX-2 and AQP3 are detected by western blotting with or without the treatment of BAY and RFP.

    BAY 11-7082 purchased from MCE. Usage Cited in: Evid-Based Compl ALlt. 2019 Jan 13;2019:9406342.  [Abstract]

    Protein expressions of AQP3, COX-2 and p-P65 are analyzed by western blot analysis with or without the treatment of BAY and RFP.

    BAY 11-7082 purchased from MCE. Usage Cited in: Cancer Lett. 2018 Aug 1;428:77-89.  [Abstract]

    Western blots show that the NF-κB inhibitors BAY11-7082, Parthenolide, and JSH-23 cause dramatic time- and dose-dependent reductions in MGMT protein expression in LN18 and T98G glioma cells.

    BAY 11-7082 purchased from MCE. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291.  [Abstract]

    Real-time PCR analysis shows that meningitic E. coli strains PCN033- and RS218-induced proinflammatory cytokines production is significantly decreased via NF-κB signaling inhibition with 10 μM of BAY11-7082.

    BAY 11-7082 purchased from MCE. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291.  [Abstract]

    Real-time PCR analysis shows that meningitic E. coli strains PCN033- and RS218-induced proinflammatory cytokines production is significantly decreased via NF-κB signaling inhibition with 10 μM of BAY11-7082.

    BAY 11-7082 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Aug 29;9(9):880.  [Abstract]

    NF-κB inhibitor Bay 11-7082 treatment inhibits the activation of SOX4 and EMT process induced by CXCL1.

    BAY 11-7082 purchased from MCE. Usage Cited in: Cell Mol Life Sci. 2018 Mar;75(6):1117-1132.  [Abstract]

    Sertoli cells (SC) are pretreated with BAY11-7082 at 2 μM for 1 h, followed by the addition of MC-LR into the culture medium. Expression of p-p65 and MMP-8 is measured by western blotting.

    BAY 11-7082 purchased from MCE. Usage Cited in: Cell Mol Life Sci. 2018 Jul;75(14):2627-2641.  [Abstract]

    RAW264.7 macrophages are pre-treated with the inhibitor of ERK, JNK, P38, P65, and AKT signal pathway.Western blot analyzes the non- and phosphorylation of ERK, JNK, P38, P65, and AKT.

    BAY 11-7082 purchased from MCE. Usage Cited in: Front Immunol. 2018 Sep 19;9:2121.  [Abstract]

    RPMI 2650 cells is incubated with or without IL-17A (100 ng/mL)+BAY 11-7082 (5 μM) for 24 h. WB is performed to measure the expression of MMP-9 protein levels.

    BAY 11-7082 purchased from MCE. Usage Cited in: Lab Invest. 2018 Apr;98(4):462-476.  [Abstract]

    Caco-2BBe cells are pretreated with BAY-11-7082 for 30 min and then treated with TNF-α (100 ng/mL) for 6 h. BAY-11-7082 treatment prevented the TNF-α-induced decrease in downregulated in adenoma (DRA).

    BAY 11-7082 purchased from MCE. Usage Cited in: Cancer Biol Ther. 2018 Mar 4;19(3):222-229.  [Abstract]

    Western blot analysis of PIK3R3, TP, p-P65, P65 expression after transfected with PIK3R3 at the presence of BAY11-7082.

    BAY 11-7082 purchased from MCE. Usage Cited in: Environ Pollut. 2017 Oct;229:964-975.  [Abstract]

    NF-κB-dependent immune responses in testicular cells. Sertoli cells (SC), Leydig cells (LC), and germ cells (GC) are pretreated with BAY11-7082 (BAY) at 2 μM for 1 h, followed by the addition of MC-LR into the culture medium to a final concentration of 1 μM and incubation for 6 h. Expression of phosphorylated NF-κB p65 (p-p65) and total p65 is measured by western blotting. The ratio of p-65/p65 is determined by densitometry and is expressed as means±SEM (n=5).

    BAY 11-7082 purchased from MCE. Usage Cited in: Eur J Med Chem. 2017 Oct 20;139:337-348.  [Abstract]

    The cells are treated with different concentrations of compound 6k, and then in the presence or absence of LPS (1 μg/mL) for 24 h. Bay 11-7082 is the NF-κB inhibitors (20 μM). The results are showed as means±SD (n=3) of at least three independent experiments.

    BAY 11-7082 purchased from MCE. Usage Cited in: Int Immunopharmacol. 2017 Sep;50:30-37.  [Abstract]

    BV-2 cells are pretreated with or without Bay11-7082 (10 μM) for 30 min, and then incubated with 20 μM Aβ1–42 for 24 h. The protein expression of TNF-α, IL-1β, and IL-6 are detected by western blot.

    BAY 11-7082 purchased from MCE. Usage Cited in: Toxicol Appl Pharmacol. 2017 Mar 22;323:44-52.  [Abstract]

    The HUVECs are subjected to NF-κB inhibitor BAY 11-7082 (10 μM) for 1 h prior to MGO (1.0 mM) treatment for 24 h, then Bcl-2/ Bax ratio are determined by western blot.

    BAY 11-7082 purchased from MCE. Usage Cited in: Int Immunopharmacol. 2016 Oct;39:149-57.  [Abstract]

    AOPPs-induced increase in catabolic factors is regulated by NF-κB activation. Chondrocytes are pretreated with or without NF-κB specific inhibitor Bay11-7082 (0.5 μM), followed by AOPPs treatment. NF-κ B p65 phosphorylation, IκB-α degradation and catabolic factors are detected by Western blot analysis. GAG production is determined by DMB method. Addition of Bay11-7082 blocks AOPPs-induced phosphorylation of NF-κB p65 and prevents degradation of IκB-α.

    查看 IKK 亚型特异性产品:

    查看所有亚型
    IKK-α IKK-β IKK IKKε TBK1

    查看 Deubiquitinase 亚型特异性产品:

    查看所有亚型
    USP1 USP2 USP7 USP8 USP30 UCHL1

    查看 NF-κB 亚型特异性产品:

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    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    • 生物活性

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    BAY 11-7082 is an IκBα phosphorylation and NF-κB inhibitor. BAY 11-7082 selectively and irreversibly inhibits the TNF-α-induced phosphorylation of IκB-α, and decreases NF-κB and expression of adhesion molecules. BAY 11-7082 inhibits ubiquitin-specific protease USP7 and USP21 (IC50=0.19, 0.96 μM, respectively). BAY 11-7082 inhibits gasdermin D (GSDMD) pore formation in liposomes and inflammasome-mediated pyroptosis and IL-1β secretion in human and mouse cells[1][2][3][4][5].

    IC50 & Target[1][2]

    NF-κB

     

    USP7

    0.19 μM (IC50)

    USP21

    0.96 μM (IC50)

    Autophagy

     

    体外研究
    (In Vitro)

    BAY 11-7082 (BAY 11-7821) 是一种 NF-κB 抑制剂，可诱导 HTLV-I 感染的 T 细胞系凋亡，但仅可忽略 HTLV-I 阴性 T 细胞的凋亡。Bay 11-7082 快速有效地降低 HTLV-I 感染的 T 细胞系中 NF-κB 的 DNA 结合，并下调受 NF-κB 调节的抗凋亡基因 Bcl-xL 的表达。Bay 11-7082 选择性抑制人 T 细胞系中 Tax 诱导的 NF-κB 活性[1]。
    BAY 11-7082 可抑制 NFκB 信号传导，最近显示可通过与催化性半胱氨酸残基共价反应来抑制大多数 E2 和 E3 连接酶。此外，BAY 11-7082 还通过与这些酶的催化性 Cys 残基反应来抑制几种酪氨酸磷酸酶。NSC 697923 最初显示可抑制 E2 连接酶 Ubc13-Uev1A[2]。
    BAY 11-7082 抑制 IκBα 的磷酸化和 NF-κB 的激活，诱导 HBL-1 细胞死亡。BAY 11-7082 完全抑制 IKKβ 激活环的 LPS 刺激和 IL-1 刺激磷酸化[3]。
    BAY 11-7082 通过抑制 TNF-α 诱导的 IκB-α 磷酸化，导致 NF-κB 减少和粘附分子表达减少[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    BAY 11-7082 相关抗体:
    体内研究
    (In Vivo)

    BAY 11-7082 (2.5 mg/kg 和 5 mg/kg；瘤内注射；每周两次，持续 21 天) 以剂量依赖性方式显著抑制肿瘤生长[6]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Four-week-old male BALB/c nude mice with human gastric carcinoma cell lines HGC-27[6]
    Dosage: Low-dose (2.5 mg/kg body weight), high-dose (5 mg/kg body weight)
    Administration: Intratumoral injection; twice-weekly for 21 days
    Result: Suppressed tumor growth in a dose-dependent manner.
    分子量

    207.25

    Formula

    C10H9NO2S
    CAS 号
    性状

    固体

    颜色

    White to off-white

    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性数据
    细胞实验： 

    DMSO 中的溶解度 : ≥ 100 mg/mL (482.51 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    * "≥" means soluble, but saturation unknown.

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 4.8251 mL 24.1255 mL 48.2509 mL
    5 mM 0.9650 mL 4.8251 mL 9.6502 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物实验：

    请根据您的 实验动物和给药方式 选择适当的溶解方案。

    以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
    ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
    以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

    • 方案 一

      请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (12.06 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

      生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
    • 方案 二

      请依序添加每种溶剂： 10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (12.06 mM); 澄清溶液

      此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。

      以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    请输入您的动物体内配方组成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。
    方案所需 助溶剂 包括：DMSO， ，均可在 MCE 网站选购。 ，Tween 80，均可在 MCE 网站选购。
    计算结果
    工作液所需浓度 : mg/mL
    储备液配制方法 : mg 药物溶于 μL  DMSO（母液浓度为 mg/mL）。
    您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。
    动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。
    连续给药周期超过半月以上，请谨慎选择该方案。
    请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热 （超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。
    纯度 & 产品资料

    纯度: 99.98%

    COA (169 KB) RP-HPLC (254 KB)

    产品使用指南 (1538 KB)
    参考文献

    BAY 11-7082 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.8251 mL 24.1255 mL 48.2509 mL 120.6273 mL
    5 mM 0.9650 mL 4.8251 mL 9.6502 mL 24.1255 mL
    10 mM 0.4825 mL 2.4125 mL 4.8251 mL 12.0627 mL
    15 mM 0.3217 mL 1.6084 mL 3.2167 mL 8.0418 mL
    20 mM 0.2413 mL 1.2063 mL 2.4125 mL 6.0314 mL
    25 mM 0.1930 mL 0.9650 mL 1.9300 mL 4.8251 mL
    30 mM 0.1608 mL 0.8042 mL 1.6084 mL 4.0209 mL
    40 mM 0.1206 mL 0.6031 mL 1.2063 mL 3.0157 mL
    50 mM 0.0965 mL 0.4825 mL 0.9650 mL 2.4125 mL
    60 mM 0.0804 mL 0.4021 mL 0.8042 mL 2.0105 mL
    80 mM 0.0603 mL 0.3016 mL 0.6031 mL 1.5078 mL
    100 mM 0.0483 mL 0.2413 mL 0.4825 mL 1.2063 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BAY 11-708219542-67-7BAY 11-7821IKKDeubiquitinaseAutophagyApoptosisNF-κBIκB kinaseI kappa B kinase DUBsNuclear factor-κBNuclear factor-kappaBphosphorylationadhesioninflammasomeubiquitinB-celllymphomaleukaemicT-cellcytokineInhibitorinhibitorinhibit

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