2. Academic Validation
  3. PSAT1 promotes autophagy to resist insufficient autophagy caused by cigarette smoke extract in human airway epithelial cells

PSAT1 promotes autophagy to resist insufficient autophagy caused by cigarette smoke extract in human airway epithelial cells

  • Toxicol In Vitro. 2023 Oct 11:105711. doi: 10.1016/j.tiv.2023.105711.
Lixing Wang 1,  Furong Yan 1,  Yongbin Shi 1,  Yifei Liu 1,  Xiaoshan Su 2,  Yaping Zhang 3
Affiliations

Affiliations

  • 1 Clinical Center for Molecular Diagnosis and Therapy, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China.
  • 2 Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Fujian Medical University, Respirology Medicine Centre of Fujian Province, Quanzhou, Fujian, China.
  • 3 Clinical Center for Molecular Diagnosis and Therapy, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China. Electronic address: icesuri@126.com.
PMID: 37832835 DOI: 10.1016/j.tiv.2023.105711
Abstract

The inhaling of cigarette smoke (CS) causes damage to airway epithelial cells, which is related to chronic obstructive pulmonary disease (COPD). It has been established that CS induces Autophagy, but it is still unclear whether excessive or insufficient Autophagy results in cell death. This study discovered that CS significantly elevates PSAT1 expression in bronchial epithelial cells. Further studies using Autophagy Inhibitor, RNA interference, RT-qPCR, western blot, and CCK-8 assay in 16-HBE cells have confirmed that Autophagy is temporarily initiated by cigarette smoke extract, but insufficient Autophagy leads to cell death. PSAT1 induced by CSE promotes Autophagy and resists insufficient Autophagy caused by CSE through Akt/mTOR pathway in human bronchial epithelial cells, playing a protective role.

Keywords

Airway epithelial cells; Autophagy; COPD; Cigarette smoke; PSAT1; mTOR.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z