2. Academic Validation
  3. Paclitaxel Inhibits Proliferation by Negatively Regulating Cdk1-Cell Cycle Axis in Rat Airway Smooth Muscle Cells

Paclitaxel Inhibits Proliferation by Negatively Regulating Cdk1-Cell Cycle Axis in Rat Airway Smooth Muscle Cells

  • J Asthma. 2024 May 2:1-10. doi: 10.1080/02770903.2024.2349599.
Bo Xiao 1 2 3 4,  Zhiheng Huang 2 3,  Liangxian Li 5,  Lixia Hou 2,  Dong Yao 1 6 7,  Biwen Mo 1 6 7
Affiliations

Affiliations

  • 1 Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Guilin Medical University, 541199, Guilin, China.
  • 2 The Laboratory of Respiratory Disease, Affiliated Hospital of Guilin Medical University, Guilin 541002, China.
  • 3 Department of Pulmonary and Critical Care Medicine, Affiliated Hospital of Guilin Medical University, Guilin 541001, China.
  • 4 Laboratory of Basic Research on Respiratory Diseases, Guangxi Health Commission, Guilin Medical University, Guilin 541199, China.
  • 5 Guangxi Key Laboratory of Brain and Cognitive Neuroscience, Guilin Medical University, Guilin 541199, China.
  • 6 Guangxi Clinical Research Center for Diabetes and Metabolic Diseases, Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, The Second Affiliated Hospital of Guilin Medical University, 541199, Guilin, China.
  • 7 Guangxi Key Laboratory of Metabolic Reprogramming and Intelligent Medical Engineering for Chronic Diseases, The key laboratory of Respiratory Diseases, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University, 541001 Guilin, China.
PMID: 38696283 DOI: 10.1080/02770903.2024.2349599
Abstract

Objective: Paclitaxel exhibits outstanding biological activities in inhibiting cell proliferation and inducing cell Apoptosis. But the effects of paclitaxel on airway smooth muscle cells (ASMCs) have not been reported yet. The purpose of this study is to determine the effects of paclitaxel on the proliferation and Apoptosis of ASMCs.

Methods: Rat primary ASMCs were isolated and used in all the experiments. Cell Counting Kit-8 (CCK-8) assay and Edu assay were used to analyse the cell viability and proliferation respectively. Flow cytometry was used to detect the cell cycle and Apoptosis. Quantitative Real-Time PCR (qRT-PCR), western blotting, and immunostaining were used to detect the expression of Cyclin-dependent kinase 1 (CDK1).

Results: Our study showed that paclitaxel inhibits the proliferation of ASMCs in a dose and time gradient dependent manner. Further study displayed that cell cycle is arrested at G2/M phase. And CDK1 was dramatically down-regulated by paclitaxel treatment. Cell morphological analysis showed that ASMCs are elliptical with a larger surface area after paclitaxel treatment. Nucleus morphological analysis showed that the nuclei are in a diffuse state after paclitaxel treatment. But paclitaxel did not induce the Apoptosis of ASMCs.

Conclusions: Our study demonstrated that paclitaxel inhibits the proliferation of ASMCs at least partly by negatively regulating Cdk1-cell cycle axis.

Keywords

ASMCs; Cdk1; Cell cycle; Cell proliferation; Paclitaxel.

Figures
Products
Inhibitors & Agonists
Other Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z