2. Academic Validation
  3. Activin A inhibits the migration of human lung adenocarcinoma A549 cells induced by EGF

Activin A inhibits the migration of human lung adenocarcinoma A549 cells induced by EGF

  • Int Immunopharmacol. 2024 Dec 5;142(Pt B):113170. doi: 10.1016/j.intimp.2024.113170.
Fenglin Zhang 1,  Xueling Cui 2,  Ke Yang 3,  Rui Guo 1,  Linjing Zhu 4,  Wei Zhao 5,  Zhonghui Liu 6,  Boyang Liu 7
Affiliations

Affiliations

  • 1 Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin Province 130021, China.
  • 2 Department of Genetics, College of Basic Medical Sciences, Jilin University, Changchun, Jilin Province 130021, China; Key Laboratory of Neuroimmunology and Clinical Immunology in Jilin Province, Jilin Province 130021, China.
  • 3 Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui Province 230031, China.
  • 4 Department of Genetics, College of Basic Medical Sciences, Jilin University, Changchun, Jilin Province 130021, China.
  • 5 Key Laboratory of Neuroimmunology and Clinical Immunology in Jilin Province, Jilin Province 130021, China; Department of Internal Medicine, The First Hospital of Jilin University, Changchun, Jilin Province 130021, China.
  • 6 Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin Province 130021, China; Key Laboratory of Neuroimmunology and Clinical Immunology in Jilin Province, Jilin Province 130021, China.
  • 7 Department of Genetics, College of Basic Medical Sciences, Jilin University, Changchun, Jilin Province 130021, China; Department of Scientific Research, Jilin Jianzhu University, Changchun, Jilin Province 130118, China. Electronic address: zhonghui@jlu.edu.cn.
PMID: 39288626 DOI: 10.1016/j.intimp.2024.113170
Abstract

Activin A, a member of the transforming growth factor β (TGF-β) superfamily, is involved in tumorigenesis and tumor progression. However, it remains unclear whether Activin A can affect the migration of lung adenocarcinoma (LUAD) cells. In this study, the results of differentially expressed genes (DEGs) identification revealed that lung adenocarcinoma tissues exhibited lower expression of activin βA mRNA, but higher expression of epidermal growth factor (EGF) and MMP9 mRNA compared to nontumor tissues. Moreover, we found that Activin A inhibited human LUAD A549 cell proliferation promoted by EGF. Additionally, EGF induced A549 cell migration in microfluidic device, while Activin A attenuated EGF actions. Simultaneously, EGF increased the levels of migration-related proteins, but Activin A played the opposite role. Furthermore, the study revealed that EGF upregulated the ratio of p-ERK/ERK in A549 cells, which was weakened by Activin A, and A549 cell migration regulated by Activin A was not related to calcium signaling. In addition, the inhibitory effect of Activin A on EGF-induced A549 cell migration was attenuated by the ERK Inhibitor FR180204. These findings demonstrate that Activin A effectively hinders the migration of A549 cells induced by EGF through ERK1/2 signaling, suggesting that targeting Activin A may hold promise in the treatment of EGF-dependent LUAD growth and metastasis.

Keywords

Activin A; Cell migration; EGF; ERK1/2 signaling; Lung adenocarcinoma; Microfluidic device.

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