15 Lipoxygenase 2 蛋白, Human (sf9)

15-Lipoxygenase 2 (15-LOX2) is a non-heme iron-containing dioxygenase crucial for peroxidation of polyunsaturated fatty acids (PUFAs). It exhibits peroxidase activity on arachidonate and linoleate, producing bioactive lipid mediators. Oxygenating arachidonyl derivatives, it generates 15-HETE-G, a peroxisome proliferator-activated receptor alpha agonist. 15-LOX2 class-switches pro-inflammatory mediators, participates in DHA oxidations to produce specialized pro-resolving mediators, and may influence cell cycle progression, proliferation, cytokine secretion, and macrophage differentiation. Importantly, it shows substrate specificity by not converting arachidonic acid to (15S)-HPETE. 15 Lipoxygenase 2 Protein, Human (sf9) is the recombinant human-derived 15 Lipoxygenase 2 protein, expressed by Sf9 insect cells , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes) mutation. The total length of 15 Lipoxygenase 2 Protein, Human (sf9) is 676 a.a., with molecular weight of 62-66 kDa.

15-脂加氧酶 2 (15-LOX2) 是一种非血红素含铁双加氧酶，在催化游离和酯化多不饱和脂肪酸 (PUFA) 的立体特异性过氧化反应中发挥着至关重要的作用，从而产生多种生物活性脂质介质。该酶对花生四烯酸具有过氧化物酶活性，产生 (15S)-氢过氧二十碳四烯酸/(15S)-HPETE，对亚油酸具有过氧化物酶活性，产生 13-氢过氧十八碳二烯酸/13-HPODE。此外，它还可以氧化花生四烯酰基衍生物，例如 2-花生四烯酰基甘油 (2-AG)，从而产生 15-羟基二十碳四烯酰基甘油 (15-HETE-G)，其充当过氧化物酶体增殖物激活受体 α 激动剂。 15-LOX2 有效地将促炎介质类别转换为抗炎中间体，并参与二十二碳六烯酸 (DHA) 的连续氧化，以生成专门的促消解介质 (SPM)，如消解素 D5。除了在脂质代谢中的作用外，15-LOX2 还可能影响细胞周期进程、细胞增殖、巨噬细胞分泌细胞因子以及巨噬细胞分化为促动脉粥样硬化泡沫细胞。重要的是，它不会将花生四烯酸转化为 15S-氢过氧二十碳四烯酸/(15S)-HPETE，突出了其底物特异性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

Tag Free

O15296 (N-G&P, M1-I676)

247  [NCBI]

62-66 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.