ABCA1 蛋白, Human (HEK293, His, Strep, FLAG)

The ABCA1 protein catalyzes the translocation of specific phospholipids from the cytoplasm to the extracellular/luminal membrane leaflet in response to ATP hydrolysis. It achieves phospholipid translocation by forcing phospholipids through its channels and loops into elongated hydrophobic tunnels in its extracellular domain. ABCA1 Protein, Human (HEK293, His, Strep, FLAG) is the recombinant human-derived ABCA1 protein, expressed by HEK293 , with N-Strep, C-Flag, N-8*His labeled tag. The total length of ABCA1 Protein, Human (HEK293, His, Strep, FLAG) is 2260 a.a., .

ABCA1 蛋白在特定磷脂从细胞质转移到膜的细胞外/腔内小叶的过程中充当关键催化剂，该过程由 ATP 水解驱动。这种活性对于磷脂向载脂蛋白的转移是不可或缺的，从而导致新生高密度脂蛋白（HDL）的形成。ABCA1 表现出比磷脂酰丝氨酸更倾向于转运磷脂酰胆碱，并且可能在细胞内胆固醇流出至载脂蛋白中发挥类似的作用，从而有助于新生 HDL 的生成。该机制涉及磷脂从质膜外表面的易位，迫使它们通过其通道和环进入其细胞外域中的细长疏水隧道。这个复杂的过程对于调节脂质稳态以及维持细胞和全身脂质平衡至关重要。

Human

HEK293

N-Strep;C-Flag;N-8*His

O95477 (A2-V2261)

19

Approximately 258.4 KDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 20 mM HEPES pH 7.5, 150 mM NaCl, 2 mM DTT, cOmplete Protease Inhibitor Tablet (Roche), 0.005% (w/v) GDN.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.