ACE2 蛋白, Human (HEK293, mFc)

ACE2 Protein, Human (HEK293, mFc) is a SARS-CoV-2 receptor. Angiotensin-Converting Enzyme 2 efficiently hydrolyses the potent vasoconstrictor angiotensin II to angiotensin. It is a consequence of this action that ACE2 participates in the renin-angiotensin system.

血管紧张素转换酶 2 (ACE2) 是一种 I 型整合膜蛋白，充当羧肽酶，从其底物的 C 末端切割单个疏水/碱性残基。ACE2 有效地将强效血管收缩剂血管紧张素 II 水解为血管紧张素。ACE2 参与肾素-血管紧张素系统就是这种作用的结果。然而，ACE2 也会水解强啡肽 A (1–13)、apelin-13 和 des-Arg9 缓激肽。ACE2 在这些肽系统中的作用尚未被揭示。ACE2 的生理作用与高血压、心脏功能、心脏功能和糖尿病有关，并且是严重急性呼吸综合征冠状病毒的受体。本文回顾了 ACE2 的生物化学，并讨论了一些重要发现，例如 ACE2 和睾丸 ACE 晶体结构的阐明以及 ACE2 抑制剂的开发，这些发现为该酶的未来研究奠定了基础。

Measured by its binding ability in a functional ELISA. Immobilized Human ACE2, at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein. The ED₅₀ for this effect is 8.465 ng/mL, corresponding to a specific activity is 1.18×10^5 Unit/mg.

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  Measured by its binding ability in a functional ELISA. Immobilized Human ACE2,at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein. The ED₅₀ for this effect is 8.465 ng/mL, corresponding to a specific activity is 1.18×10⁵ Unit/mg.

Human

HEK293

C-mFc

Q9BYF1-1 (Q18-S740)

59272  [NCBI]

QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS

110-140 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 300 mM NaCl, 1 mM ZnCl₂, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Angiotensin-converting enzyme 2 (ACE2) as a SARS-CoV-2 receptor: molecular mechanisms and potential therapeutic target.

  [2]. Warner FJ, et al. Angiotensin-converting enzyme-2: a molecular and cellular perspective. Cell Mol Life Sci. 2004 Nov;61(21):2704-13.  [Content Brief]