ACE2 蛋白, Mouse (HEK293, His-Fc)

ACE2 protein is an important carboxypeptidase in the renin-angiotensin system, which can regulate blood volume and vascular resistance and maintain cardiovascular homeostasis. It converts angiotensin I to the antihypertrophic angiotensin 1-9 and angiotensin II to the vasodilatory angiotensin 1-7, thereby opposing vasoconstriction. ACE2 Protein, Mouse (HEK293, His-Fc) is the recombinant mouse-derived ACE2 protein, expressed by HEK293 , with C-hFc, C-His, C-8*His labeled tag. The total length of ACE2 Protein, Mouse (HEK293, His-Fc) is 723 a.a., with molecular weight of ~130 kDa.

ACE2 是肾素-血管紧张素激素系统中一种重要的反调节羧肽酶，是血容量和全身血管阻力的关键调节因子，有助于心血管稳态。其酶活性包括将血管紧张素 I 转化为血管紧张素 1-9（一种在心肌细胞中具有抗肥大作用的肽），以及将血管紧张素 II 转化为血管紧张素 1-7（以其血管舒张和抗增殖特性而闻名），从而抵消血管紧张素的血管收缩作用。血管紧张素II。ACE2 进一步在氨基酸转运中发挥作用，作为氨基酸转运蛋白 SLC6A19 的结合伴侣，调节其细胞表面运输和活性。此外，ACE2 有助于调节各种血管活性肽，例如神经降压素、运动紧张素和去精氨酸缓激肽，以及其他生物肽，例如 apelins、酪啡肽和强啡肽 A。其多方面功能使 ACE2 成为心血管疾病的关键参与者。和氨基酸稳态。

Measured by its binding ability in a functional ELISA. Immobilized Mouse ACE2, at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein. The ED₅₀ for this effect is 149.1 ng/mL, corresponding to a specific activity is 6706.9 Unit/mg.

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  Measured by its binding ability in a functional ELISA. Immobilized Mouse ACE2, at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein. The ED₅₀ for this effect is 149.1 ng/mL, corresponding to a specific activity is 6706.9 Unit/mg.

Mouse

HEK293

C-8*His;C-hFc

Q8R0I0-1 (Q18-T740)

70008  [NCBI]

QSLTEENAKTFLNNFNQEAEDLSYQSSLASWNYNTNITEENAQKMSEAAAKWSAFYEEQSKTAQSFSLQEIQTPIIKRQLQALQQSGSSALSADKNKQLNTILNTMSTIYSTGKVCNPKNPQECLLLEPGLDEIMATSTDYNSRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYNDYGDYWRGDYEAEGADGYNYNRNQLIEDVERTFAEIKPLYEHLHAYVRRKLMDTYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTVPFAQKPNIDVTDAMMNQGWDAERIFQEAEKFFVSVGLPHMTQGFWANSMLTEPADGRKVVCHPTAWDLGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYARQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLPSDFQEDSETEINFLLKQALTIVGTLPFTYMLEKWRWMVFRGEIPKEQWMKKWWEMKREIVGVVEPLPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKYNGSLHKCDISNSTEAGQKLLKMLSLGNSEPWTKALENVVGARNMDVKPLLNYFQPLFDWLKEQNRNSFVGWNTEWSPYADQSIKVRISLKSALGANAYEWTNNEMFLFRSSVAYAMRKYFSIIKNQTVPFLEEDVRVSDLKPRVSFYFFVTSPQNVSDVIPRSEVEDAIRMSRGRINDVFGLNDNSLEFLGIHPTLEPPYQPPVT

Approximately 130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 1 mM ZnCl₂, pH 7.5 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.