Animal-Free BMP-8a 蛋白, Human (His)

BMP-8 is a pleiotropic ligand protein act as a reproductive system regulator, enriched in the ovary. BMP-8 is encoded by a pair of genes BMP8A and BMP8B, belonging to TNF-β family^[1]. BMP-8A activates the SMAD1/5/8 and the SMAD2/3 pathways in granulosa cells, to inhibit gonadotropin-induced progesterone production and steroidogenesis-related gene expression^[1]. BMP-8A also involves in Nrf2 phosphorylation in cancer cells to promote survival and drug resistance^[2]. BMP-8a Protein, Human is 402 a.a. with 2 glycosylation domains. Animal-Free BMP-8a Protein, Human (His) is a animal free recombinant human protein produced in E. coli cells, with 139 a.a. (A264-H402) and C-terminal His-tag.

“BMP-8 是一种多效性配体蛋白，充当生殖系统调节剂。BMP-8 由属于 TNF-β 家族的一对基因 BMP8A 和 BMP8B 编码。GMP-8 通过与I 型受体 BMPR1A 和 II 型受体 BMPR2。BMP8A 和 BMP8B 均在卵巢中富集，并激活不同细胞（包括精原细胞、P19 和 293T 细胞）中的经典 BMP 信号传导^[1]。BMP-8 是广泛存在于不同动物中，而人类的BMP-8A和BMP-8B序列与小鼠差异较大，相似度分别为85.96%和74.44%。
至于BMP8A，主要由颗粒细胞分泌在生长的卵泡内。BMP8A 编码 TGF-β 蛋白超家族的分泌配体，以结合各种 TGF-β 受体，导致 SMAD 家族转录因子的募集和激活并调节基因表达。编码的前原蛋白经过蛋白水解加工，生成每个二硫键连接的同二聚体^[2]的亚基。BMP-8A 蛋白激活颗粒细胞中的 SMAD1/5/8 和 SMAD2/3 通路，抑制促性腺激素诱导的孕激素产生和类固醇生成相关基因的表达^[1]。BMP8A 蛋白通过激活精原细胞中的 SMAD1/5/9 和 SMAD2/3 来维持精子发生，从而在生殖系统的发育中发挥作用。BMP-8A 蛋白还可激活透明细胞肾细胞癌 (ccRCC) 中的 Nrf2 和 Wnt 通路，促进细胞增殖并抑制细胞凋亡^[2]。
至于 BMP8B，该蛋白由透明细胞肾细胞癌 (ccRCC) 分泌棕色/米色脂肪细胞并增强能量耗散，作为 AT 神经血管重塑的相互关联调节器，并且是肥胖症的潜在目标^[3]。BMP8B 通过中枢和外周作用增加棕色脂肪组织的产热^[4]。因此，BMP8B 有助于肾上腺素诱导的脂肪组织中神经血管网络的重塑，因此通过脂肪细胞 1) 分泌神经调节蛋白-4 (NRG4)，其在体外促进交感神经轴突生长和分支，2) 诱导促进血管萌芽的血管生成转录和分泌特征^[3]。BMP8B 还参与 caspase-3 和 -9 的激活以及细胞凋亡，从而抑制胰腺癌细胞的生长^[5]。
"

BMP-8a (5 ng/mL; 6 d) can be used for hHEC lines differentiation accompanied with 5 ng/mL BMP-4^[6].
BMP-8a (5 ng/mL; 6 d) can be used for PGCLC cells differentiation accompanied with 5 ng/mL BMP-4^[7].

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is 10-19.4 ng/mL.

Human

E. coli

C-His

AAP74559.1 (A264-H402)

353500  [NCBI]

MAVRPLRRRQPKKSNELPQANRLPGIFDDVHGSHGRQVCRRHELYVSFQDLGWLDWVIAPQGYSAYYCEGECSFPLDSCMNATNHAILQSLVHLMKPNAVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCH

Approximately 16.61 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5/pH 4.5.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wu FJ, et al. Human BMP8A suppresses luteinization of rat granulosa cells via the SMAD1/5/8 pathway. Reproduction. 2020 Mar;159(3):315-324.  [Content Brief]

  [2]. Yu YP, et al. BMP8A promotes survival and drug resistance via Nrf2/TRIM24 signaling pathway in clear cell renal cell carcinoma. Cancer Sci. 2020 May;111(5):1555-1566.  [Content Brief]

  [3]. Pellegrinelli V, et al. Adipocyte-secreted BMP8b mediates adrenergic-induced remodeling of the neuro-vascular network in adipose tissue. Nat Commun. 2018 Nov 26;9(1):4974.  [Content Brief]

  [4]. Whittle AJ, et al. BMP8B increases brown adipose tissue thermogenesis through both central and peripheral actions. Cell. 2012 May 11;149(4):871-85.  [Content Brief]

  [5]. Cheng Z, et al. BMP8B mediates the survival of pancreatic cancer cells and regulates the progression of pancreatic cancer. Oncol Rep. 2014 Nov;32(5):1861-6.  [Content Brief]

  [6]. Yan A, et al. DAZL regulates proliferation of human primordial germ cells by direct binding to precursor miRNAs and enhances DICER processing activity. Nucleic Acids Res. 2022 Oct 28;50(19):11255-11272.  [Content Brief]

  [7]. Hayashi K, et al. Stepwise differentiation from na茂ve state pluripotent stem cells to functional primordial germ cells through an epiblast-like state. Methods Mol Biol. 2013;1074:175-83.  [Content Brief]