1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Animal-Free Enterokinase 蛋白, Bovine (His)

Animal-Free Enterokinase 蛋白, Bovine (His)

目录号: HY-P7341AF
产品使用指南

肠激酶蛋白在启动胰腺蛋白水解酶(包括胰蛋白酶、胰凝乳蛋白酶和羧肽酶 A)的激活中发挥关键作用。其催化功能将胰蛋白酶原转化为胰蛋白酶,引发胰凝乳蛋白酶原、羧肽酶原和弹性蛋白原的级联激活。Animal-Free Enterokinase 蛋白, Bovine (His) 是重组的 肠激酶,由 P. pastoris 表达,带有 C-His 标签。Animal-Free Enterokinase 蛋白, Bovine (His) 全长 235 个氨基酸

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

Enterokinase proteins play a key role in initiating the activation of pancreatic proteolytic enzymes, including trypsin, chymotrypsin, and carboxypeptidase A. Its catalytic function converts trypsinogen to trypsin, triggering the cascade activation of chymotrypsinogen, procarboxypeptidase, and tropoelastin. Animal-Free Enterokinase Protein, Bovine (His) is the recombinant bovine-derived Enterokinase, expressed by P. pastoris , with C-His labeled tag. The total length of Animal-Free Enterokinase Protein, Bovine (His) is 235 a.a.,

研究背景

肠激酶蛋白在胰腺蛋白水解酶原(特别是胰蛋白酶、胰凝乳蛋白酶和羧肽酶 A)的激活过程启动中发挥着关键作用。其催化功能驱动胰蛋白酶原转化为胰蛋白酶,引发一系列激活,并延伸到其他酶原,例如胰蛋白酶原。如胰凝乳蛋白酶原、羧肽酶原和弹性蛋白酶原。通过催化这些转化,肠激酶在胰腺蛋白水解途径的复杂网络中充当关键介质,确保蛋白质消化所涉及的必需酶的及时和协调激活。

种属

Bovine

表达系统

P. pastoris

标签

C-His

蛋白编号

P98072-1 (I801-H1035)

基因 ID

282009

同用名
Enteropeptidase; Enterokinase; Serine protease 7; Transmembrane protease serine 15; Enteropeptidase; ENTK; PRSS7
氨基酸序列

/

分子量

Approximately 40 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

< 1 EU/μg of protein by gel clotting method

文件资料

Animal-Free Enterokinase Protein, Bovine (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Animal-Free Enterokinase Protein, Bovine (His)
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HY-P7341AF
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