Animal-Free IL-15 蛋白, Mouse (His)

The IL-15 protein is a cytokine that crucially shapes inflammatory and protective immune responses by regulating innate and adaptive immune cells. It stimulates the proliferation and activation of natural killer cells, T cells and B cells, and promotes the secretion of cytokines. Animal-Free IL-15 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-15 protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free IL-15 Protein, Mouse (His) is 114 a.a., with molecular weight of ~14.06 kDa.

IL-15 蛋白是一种细胞因子，在针对微生物入侵者和寄生虫的炎症和保护性免疫反应的发展中发挥着至关重要的作用。它通过调节先天免疫系统和适应性免疫系统的免疫细胞来实现这一目标。IL-15刺激自然杀伤细胞、T细胞和B细胞的增殖和活化，同时还促进各种细胞因子的分泌。在单核细胞中，IL-15 诱导趋化因子 IL8 和单核细胞趋化蛋白 1/CCL2 的产生，从而将中性粒细胞和单核细胞吸引到感染部位。与大多数细胞因子不同，IL-15 在产生 IL-15 的细胞表面表达，与其高亲和力 IL15RA 相关，向表达 IL2RB 和 IL2RG 受体亚基的靶细胞传递信号。这种结合触发 JAK1 和 JAK3 的磷酸化，募集并随后磷酸化转录 3/STAT3 和 STAT5 的信号转导子和激活子。此外，在肥大细胞中，IL-15 快速磷酸化 STAT6，从而控制肥大细胞的存活和 IL4 等细胞因子的释放。

Measure by its abilit to induce CTLL-2 cells proliferation.The ED₅₀ for this effect is < 10 ng/mL.The specific activity of recombinant mousel L-15 is approximately> 1x10⁵ IU/mg.

Mouse

E. coli

N-His

P48346 (N49-S162)

16168  [NCBI]

NWIDVRYDLEKIESLIQSIHIDTTLYTDSDFHPSCKVTAMNCFLLELQVILHEYSNMTLNETVRNVLYLANSTLSSNKNVAESGCKECEELEEKTFTEFLQSFIRIVQMFINTS

Approximately 14.06 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 1X PBS, pH 7.4 or Proleukin buffer, pH 7.5.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.