APT-1/LYPLA1 蛋白, Human (His)

APT-1/LYPLA1 protein, an acyl-protein thioesterase, hydrolyzes fatty acids from S-acylated cysteine residues in proteins, including G alpha proteins and HRAS. It also depalmitoylates KCNMA1 and potentially ADRB2, while functioning as a lysophospholipase, hydrolyzing lysophosphatidylcholine (lyso-PC) and other lysophospholipids (lyso-PE, lyso-PI, lyso-PS). APT-1/LYPLA1 significantly influences blood coagulation by cleaving plasma phospholipids, generating lysophospholipids for ENPP2, leading to lysophosphatidic acid (LPA) production. APT-1/LYPLA1 Protein, Human (His) is the recombinant human-derived APT-1/LYPLA1 protein, expressed by E. coli, with N-6*His labeled tag. The total length of APT-1/LYPLA1 Protein, Human (His) is 230 a.a., with molecular weight of ~25.0 kDa.

APT-1/LYPLA1 蛋白充当酰基蛋白硫酯酶，催化蛋白质（包括三聚 G α 蛋白和 HRAS）内 S-酰化半胱氨酸残基的脂肪酸水解。此外，它还对 KCNMA1 和潜在的 ADRB2 具有去棕榈酰化活性。作为溶血磷脂酶，APT-1/LYPLA1 可水解溶血磷脂酰胆碱 (lyso-PC) 和其他溶血磷脂，如 lyso-PE、lyso-PI 和 lyso-PS，对硫酯酶活性具有更高的亲和力。该蛋白通过识别和裂解血浆磷脂，产生作为 ENPP2 底物的溶血磷脂，最终产生溶血磷脂酸 (LPA)，从而显着促进血液凝固。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

O75608 (M1-D230)

10434  [NCBI]

MCGNNMSTPLPAIVPAARKATAAVIFLHGLGDTGHGWAEAFAGIRSSHIKYICPHAPVRPVTLNMNVAMPSWFDIIGLSPDSQEDESGIKQAAENIKALIDQEVKNGIPSNRIILGGFSQGGALSLYTALTTQQKLAGVTALSCWLPLRASFPQGPIGGANRDISILQCHGDCDPLVPLMFGSLTVEKLKTLVNPANVTFKTYEGMMHSSCQQEMMDVKQFIDKLLPPIDHHHHHH

Approximately 25.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 100 mM NaCl, 1 Mm DTT, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.