1. 重组蛋白
  2. Immune Checkpoint Proteins CD Antigens
  3. Inhibitory Checkpoint Molecules Macrophage CD Proteins
  4. BTLA BTLA/CD272
  5. BTLA/CD272 蛋白, Human (HEK293, Fc)

BTLA/CD272 蛋白, Human (HEK293, Fc)

目录号: HY-P7679
产品使用指南 技术支持

BTLA/CD272 蛋白, Human (HEK293, Fc) 由 HEK293 细胞表达产生的带有 C-Fc 标签的重组人类 BTLA/CD272。BTLA/CD272 是免疫球蛋白超家族的一个抑制分子,参与免疫应答的抑制。

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描述

BTLA/CD272 Protein, Human (HEK293, Fc) is a recombinant human BTLA/CD272 produced in HEK293 cells, with an Fc fragment at the C-terminus. BTLA/CD272 is a suppressor molecule belonging to the immunoglobulin superfamily which is involved in the inhibition of immune responses[1].

研究背景

和 T 淋巴细胞衰减因子 (BTLA/CD272) 是共抑制受体的第三个成员,属于 CD28 免疫球蛋白超家族。BTLA/CD272 在其胞质区域包含 2 个基于酪氨酸的免疫受体抑制基序 (ITIM),并在多种造血细胞上表达,包括 T 和 B 淋巴细胞、NKT 细胞、NK 细胞、巨噬细胞、树突状细胞和滤泡 T 辅助 T 细胞[1][2]

种属

Human

表达系统

HEK293

标签

C-Fc

蛋白编号

Q7Z6A9-2 (K31-L150)

基因 ID
蛋白结构
N-term
BTLA (K31-L150)
Accession # Q7Z6A9-2
Fc
C-term
同用名
rHuBTLA/CD272, C-Fc; B- and T-Lymphocyte Attenuator; CD272; BTLA
氨基酸序列

KESCDVQLYIKRQSEHSILAGDPFELECPVKYCANRPHVTWCKLNGTTCVKLEDRQTSWKEEKNISFFILHFEPVLPNDNGSYRCSANFQSNLIESHSTTLYVTGKQNELSDTAGREINL-Fctag

分子量

Approximately 55.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BTLA/CD272 Protein, Human (HEK293, Fc)
目录号:
HY-P7679
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