CD300LF 蛋白, Mouse (HEK293, Fc)

CD300LF protein, also known as DEC-205/CD205, directs antigens to an antigen-processing compartment. It inhibits myeloid and mast cells, promoting phagocytosis of apoptotic cells. It maintains immune homeostasis by enhancing macrophage-mediated efferocytosis and inhibiting dendritic cell-mediated efferocytosis. It binds to ceramide ligands, regulating mast cell activation and allergic responses. It enhances IL-4 and IL-13 signaling and inhibits Toll-like receptor signaling and osteoclast formation. It induces macrophage cell death, acts as a receptor for murine norovirus, and determines viral tropism. CD300LF Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived CD300LF protein, expressed by HEK293, with C-mFc labeled tag. The total length of CD300LF Protein, Mouse (HEK293, Fc) is 174 a.a., with molecular weight of ~45.3 KDa.

CD300LF 蛋白，也称为 DEC-205/CD205 蛋白，作为内吞受体，负责将捕获的抗原引导至专门的抗原处理区室。据观察，它作为骨髓细胞和肥大细胞的抑制性受体，通过识别和结合凋亡细胞表面的磷脂酰丝氨酸（PS）配体，正向调节凋亡细胞的吞噬作用。CD300LF 通过促进巨噬细胞介导的胞吞作用同时抑制树突状细胞介导的胞吞作用，在维持免疫稳态中发挥着至关重要的作用。此外，它还通过与神经酰胺配体结合来负调节肥大细胞活化和过敏反应。它还可以作为白细胞介素 4 (IL-4) 的辅助受体，增强 IL-4 和 IL-13 诱导的信号传导。CD300LF 负向调节 Toll 样受体信号传导并抑制破骨细胞形成。此外，它在接触后诱导巨噬细胞死亡，并充当鼠诺如病毒 (MNV) 的功能性受体，介导病毒进入和复制，同时确定 MNV 物种向性并使非鼠哺乳动物细胞易受 MNV 感染。

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED₅₀ for this effect <11.3 μg/mL, corresponding to a specific activity is >88 U/mg.

Mouse

HEK293

C-mFc

Q6SJQ7 (E20-S193)

246746  [NCBI]

EDPVTGPEEVSGQEQGSLTVQCRYTSGWKDYKKYWCQGVPQRSCKTLVETDASEQLVKKNRVSIRDNQRDFIFTVTMEDLRMSDAGIYWCGITKGGLDPMFKVTVNIGPAIQVPITVPTMPPITSTTTIFTVTTTVKETSMFPTLTSYYSDNGHGGGDSGGGEDGVGDGFLDLS

Approximately 45.3 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.