UNC5B 蛋白, Human (HEK293, His)

UNC5B is an important netrin receptor that mediates axonal repulsion during nervous system development and binds to DCC to trigger signaling pathways. It negatively regulates vascular branching in angiogenesis, inducing tip cell filopodia retraction. UNC5B Protein, Human (HEK293, His) is the recombinant human-derived UNC5B protein, expressed by HEK293 , with C-His labeled tag. The total length of UNC5B Protein, Human (HEK293, His) is 337 a.a., with molecular weight of ~39.2 kDa.

UNC5B 是 netrin 的重要受体，在轴突引导中发挥着关键作用，主要在配体结合后的神经系统发育过程中介导神经元生长锥中的轴突排斥。这种排斥效应被认为是通过其与 DCC 的关联而精心策划的，可能触发导致轴突排斥的信号通路。除了在神经发育中的作用之外，UNC5B 还作为 netrin 受体发挥作用，对血管生成过程中的血管分支产生负面调节影响，促进内皮生长锥上的尖端细胞丝状伪足响应 netrin 的收缩。值得注意的是，UNC5B 还充当依赖性受体，在不与 netrin 配体结合时诱导细胞凋亡，这一过程涉及 DAPK1 的激活。在缺乏 netrin 的情况下，UNC5B 通过减少 DAPK1 的自抑制磷酸化来激活 DAPK1，从而增强其催化活性。该受体以上下文依赖性方式参与多种蛋白质相互作用，包括 DCC、GNAI2、FLRT3、FLRT2 和 FLRT3 的细胞质部分，强调了其在轴突引导、血管生成调节和细胞凋亡信号传导中的多方面作用。

Measured by its binding ability in a functional ELISA. Immobilized UNC5B at 5 µg/mL (100 µL/well) can bind rmNetrin-1. The ED₅₀ for this effect is 306.6 ng/mL.

Human

HEK293

C-His

Q8IZJ1 (G27-P363)

219699  [NCBI]

GTDSGSEVLPDSFPSAPAEPLPYFLQEPQDAYIVKNKPVELRCRAFPATQIYFKCNGEWVSQNDHVTQEGLDEATGLRVREVQIEVSRQQVEELFGLEDYWCQCVAWSSAGTTKSRRAYVRIAYLRKNFDQEPLGKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPTQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQAFQKTACTTICPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCMQNKKTLSDP

Approximately 48-52 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.