CD36 蛋白, Human (HEK293, Fc )

CD36 is a multifunctional glycoprotein that serves as a receptor for a variety of ligands, including thrombospondin and oxidized low-density lipoprotein. Ligand induces CD36 clusters, initiating signal transduction and internalization. CD36 Protein, Human (HEK293, Fc ) is the recombinant human-derived CD36 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD36 Protein, Human (HEK293, Fc ) is 410 a.a., with molecular weight of 90-130 kDa.

CD36 是一种多功能糖蛋白，可作为多种配体的受体，包括血小板反应蛋白、纤连蛋白、胶原蛋白或β淀粉样蛋白等蛋白质实体，以及氧化低密度脂蛋白 (oxLDL)、阴离子磷脂等脂质成分。长链脂肪酸和细菌二酰化脂肽。这些配体具有多价性，可以同时与多个受体结合，促进 CD36 簇的形成，从而启动信号转导和受体-配体复合物的内化。对辅助受体信号传导的依赖性明显是配体特异性的。细胞对这些配体的反应在肠道内的血管生成、炎症反应、脂肪酸代谢、味觉和膳食脂肪加工中发挥着关键作用。此外，CD36 结合长链脂肪酸，促进其转运到细胞中并参与肌肉脂质利用、脂肪能量储存和肠道脂肪吸收。从机制上讲，脂肪酸结合会激活下游激酶 LYN，磷酸化棕榈酰转移酶 ZDHHC5，并导致 CD36 去棕榈酰化和小凹胞吞作用。在小肠中，CD36 可能通过激活 MAPK1/3 (ERK1/2) 信号通路在膳食脂肪酸和胆固醇的近端吸收中发挥作用。它还涉及口腔脂肪的感知和偏好，介导味觉受体细胞中细胞内钙水平的增加。此外，CD36 是长链脂肪酸腹内侧下丘脑神经元感知以及能量和葡萄糖稳态调节的重要因素。作为血小板反应蛋白的受体，CD36 介导其抗血管生成作用，并与 THBS1 合作，响应游离脂肪酸升高而诱导足细胞凋亡。作为 TLR4:TLR6 异二聚体的辅助受体，CD36 在配体结合后促进单核细胞/巨噬细胞的炎症，通过 NLRP3 炎症小体的启动和激活，导致 NF-κ-B 依赖性细胞因子的产生和 IL1B 的分泌。此外，CD36 作为微生物二酰化脂肽的选择性和非冗余传感器，触发 NF-κ-B 依赖性 TNF 产生，并随后通过 TLR2:TLR6 异二聚体信号传导靶向脂筏依赖性途径中的高尔基体。在微生物感染的情况下，CD36 直接介导恶性疟原虫寄生的红细胞的细胞粘附和颗粒的内化，与 TLR 信号传导无关。

Measured by its binding ability in a functional ELISA. When Recombinant Human CD36 is immobilized at 2 µg/mL (100 µL/well) can bind Recombinant Human TSP-2. The ED₅₀ for this effect is 53.53 ng/mL.

Human

HEK293

C-hFc

P16671-1 (G30-N439)

948  [NCBI]

GDLLIQKTIKKQVVLEEGTIAFKNWVKTGTEVYRQFWIFDVQNPQEVMMNSSNIQVKQRGPYTYRVRFLAKENVTQDAEDNTVSFLQPNGAIFEPSLSVGTEADNFTVLNLAVAAASHIYQNQFVQMILNSLINKSKSSMFQVRTLRELLWGYRDPFLSLVPYPVTTTVGLFYPYNNTADGVYKVFNGKDNISKVAIIDTYKGKRNLSYWESHCDMINGTDAASFPPFVEKSQVLQFFSSDICRSIYAVFESDVNLKGIPVYRFVLPSKAFASPVENPDNYCFCTEKIISKNCTSYGVLDISKCKEGRPVYISLPHFLYASPDVSEPIDGLNPNEEEHRTYLDIEPITGFTLQFAKRLQVNLLVKPSEKIQVLKNLKRNYIVPILWLNETGTIGDEKANMFRSQVTGKIN

Approximately 90-130 kDa due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.