CD36 蛋白, Mouse (Biotinylated, HEK293, Avi-His)

CD36 is a multifunctional glycoprotein that binds to proteins such as thrombospondin, fibronectin, collagen, beta-amyloid and oxidized low-density lipoprotein, as well as lipid molecules such as anionic phospholipids and long-chain phospholipids. CD36 regulates angiogenesis, inflammatory responses, fatty acid metabolism, taste, dietary fat processing, and acts as a TLR4:TLR6 heterodimer coreceptor to mediate inflammatory responses, regulate immune responses and bacterial absorption. CD36 Protein, Mouse (Biotinylated, HEK293, Avi-His) is the recombinant mouse-derived CD36 protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag. The total length of CD36 Protein, Mouse (Biotinylated, HEK293, Avi-His) is 410 a.a., with molecular weight of 85-120 kDa.

CD36 是一种多功能糖蛋白，可作为多种配体的受体，包括血小板反应蛋白、纤连蛋白、胶原蛋白、β 淀粉样蛋白等蛋白质实体和氧化低密度脂蛋白 (oxLDL)、阴离子磷脂、长链磷脂等脂质分子。链脂肪酸和细菌二酰化脂肽。这些配体的结合启动信号转导和受体-配体复合物的内化，反应以配体特异性方式变化。CD36 的参与涉及肠道内的血管生成、炎症反应、脂肪酸代谢、味觉和膳食脂肪加工。糖蛋白结合长链脂肪酸，促进其细胞运输并参与肌肉脂质利用、脂肪能量储存和肠道脂肪吸收等过程。从机制上讲，脂肪酸结合激活下游激酶 LYN，导致 CD36 去棕榈酰化和小凹胞吞作用。CD36 在口腔脂肪感知中也发挥着关键作用，影响偏好，并在检测到舌头中的长链脂肪酸后导致胰胆分泌物的变化。此外，它有助于调节下丘脑腹内侧的能量和葡萄糖稳态，并作为血小板反应蛋白的受体，介导抗血管生成作用。CD36 作为 TLR4:TLR6 异二聚体的辅助受体，可促进单核细胞/巨噬细胞的炎症，对 oxLDL 或淀粉样蛋白 -β 等配体做出反应。此外，CD36 作为微生物二酰化脂肽的选择性传感器，触发 NF-κ-B 依赖性细胞因子的产生并参与对感染（包括结核分枝杆菌和恶性疟原虫）的反应。它还介导某些细菌的吸收，显示出其在各种生理过程中作为多功能且不可或缺的组成部分的作用。

Mouse

HEK293

C-Avi;C-6*His

Q08857 (G30-K439)

12491  [NCBI]

GDMLIEKTIKREVVLEEGTTAFKNWVKTGTTVYRQFWIFDVQNPDDVAKNSSKIKVKQRGPYTYRVRYLAKENITQDPEDHTVSFVQPNGAIFEPSLSVGTEDDNFTVLNLAVAAAPHIYQNSFVQVVLNSLIKKSKSSMFQTRSLKELLWGYKDPFLSLVPYPISTTVGVFYPYNDTVDGVYKVFNGKDNISKVAIIESYKGKRNLSYWPSYCDMINGTDAASFPPFVEKSRTLRFFSSDICRSIYAVFGSEIDLKGIPVYRFVLPANAFASPLQNPDNHCFCTEKVISNNCTSYGVLDIGKCKEGKPVYISLPHFLHASPDVSEPIEGLHPNEDEHRTYLDVEPITGFTLQFAKRLQVNILVKPARKIEALKNLKRPYIVPILWLNETGTIGDEKAEMFKTQVTGKIK

85-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.