Coagulation Factor XIV/PROC 蛋白, Human (HEK293, His)

Coagulation Factor XIV/PROC is a vital vitamin K-dependent serine protease regulating blood coagulation. Active Protein C inactivates factors Va and VIIIa in the presence of calcium ions and phospholipids, maintaining the intricate balance of coagulation pathways. Its protective effect on endothelial cell barrier function underscores a broader impact on vascular integrity. Coagulation Factor XIV/PROC Protein, Human (HEK293, His) is the recombinant human-derived Coagulation Factor XIV/PROC protein, expressed by HEK293 , with C-His labeled tag. The total length of Coagulation Factor XIV/PROC Protein, Human (HEK293, His) is 443 a.a., with molecular weight of 56-64 & 45 kDa, respectively.

凝血因子 XIV (PROC) 是一种维生素 K 依赖性丝氨酸蛋白酶，对于调节凝血至关重要。在钙离子和磷脂存在的情况下，蛋白 C 的活性形式在灭活因子 Va 和 VIIIa 方面发挥着至关重要的作用，对凝血途径的复杂平衡做出了重大贡献。此外，蛋白 C 对内皮细胞屏障功能具有保护作用，突显其对血管完整性的更广泛影响。

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (HY-137784)that incubate at the room temperature in kinetic mode for 5 minutes. The specific activity is 44.7-98.851 pmol/min/μg.

Human

HEK293

C-His

P04070 (T19-P461)

5624  [NCBI]

TPAPLDSVFSSSERAHQVLRIRKRANSFLEELRHSSLERECIEEICDFEEAKEIFQNVDDTLAFWSKHVDGDQCLVLPLEHPCASLCCGHGTCIDGIGSFSCDCRSGWEGRFCQREVSFLNCSLDNGGCTHYCLEEVGWRRCSCAPGYKLGDDLLQCHPAVKFPCGRPWKRMEKKRSHLKRDTEDQEDQVDPRLIDGKMTRRGDSPWQVVLLDSKKKLACGAVLIHPSWVLTAAHCMDESKKLLVRLGEYDLRRWEKWELDLDIKEVFVHPNYSKSTTDNDIALLHLAQPATLSQTIVPICLPDSGLAERELNQAGQETLVTGWGYHSSREKEAKRNRTFVLNFIKIPVVPHNECSEVMSNMVSENMLCAGILGDRQDACEGDSGGPMVASFHGTWFLVGLVSWGEGCGLLHNYGVYTKVSRYLDWIHGHIRDKEAPQKSWAP

Approximately 40-48 & 56-80 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.