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  3. COX4NB 蛋白, Human (His)

COX4NB 蛋白, Human (His)

目录号: HY-P77342
产品使用指南

COX4NB蛋白是内质网膜蛋白复合物(EMC)的组成部分,能够以能量独立的方式将新合成的膜蛋白插入内质网膜。它对具有弱疏水性或不稳定特征的跨膜结构域的偏好指导了共翻译和翻译后插入过程。COX4NB 蛋白, Human (His) 是重组的 COX4NB 蛋白,由 E. coli 表达,带有 N-His 标签。COX4NB 蛋白, Human (His) 全长 210 个氨基酸,分子量约为 ~27 kDa。

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描述

COX4NB protein is a component of the endoplasmic reticulum membrane protein complex (EMC) and is able to insert newly synthesized membrane proteins into the endoplasmic reticulum membrane in an energy-independent manner. Its preference for transmembrane domains with weakly hydrophobic or unstable characteristics guides co- and post-translational insertion processes. COX4NB Protein, Human (His) is the recombinant human-derived COX4NB protein, expressed by E. coli , with N-His labeled tag. The total length of COX4NB Protein, Human (His) is 210 a.a., with molecular weight of ~27 kDa.

研究背景

COX4NB 蛋白是内质网膜蛋白复合物 (EMC) 的重要组成部分,有助于新合成的膜蛋白以能量独立的方式插入内质网 (ER) 膜。它表现出偏好容纳具有弱疏水性或含有不稳定特征(例如带电和芳香残基)的跨膜结构域的蛋白质。COX4NB 积极参与共翻译和翻译后插入过程,在多次传递膜蛋白的共翻译插入中发挥关键作用,其中停止转移膜锚定序列转化为内质网跨膜螺旋。此外,它对于将尾锚定 (TA) 蛋白翻译后插入内质网膜中至关重要。通过介导 N-exo 拓扑中 N 端跨膜结构域的正确共翻译插入,将易位的 N 端定位在 ER 腔中,COX4NB 控制多通道膜蛋白(包括 G 蛋白偶联受体)的拓扑。COX4NB 通过其在蛋白质膜插入中的调节作用间接影响各种细胞过程,是 EMC 复合体的重要组成部分,与 EMC8 和 EMC9 等其他亚基协同发挥作用。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

O43402-1 (M1-C210)

基因 ID
蛋白结构
N-term
His
COX4NB (M1-C210)
Accession # O43402-1
C-term
同用名
ER membrane protein complex subunit 8; Neighbor of COX4; C16orf2; COX4AL; COX4NB; FAM158B; NOC4
分子量

Approximately 27 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

COX4NB Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
COX4NB Protein, Human (His)
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HY-P77342
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