DCD 蛋白, Human (P.pastoris, His)

The DCD protein is found in sweat and has antimicrobial activity against Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, and Candida albicans. DCD Protein, Human (P.pastoris, His) is the recombinant human-derived DCD protein, expressed by P. pastoris , with N-6*His labeled tag.

DCD 蛋白存在于汗液中，在细菌定植的早期阶段表现出抗菌活性。分泌的肽形成能够与病原体膜结合并插入其中的同源六聚体复合物。DCD 蛋白插入细菌膜后会产生阴离子通道，可能会改变对细菌生存至关重要的跨膜电位。它对多种病原体表现出高效能，包括大肠杆菌、粪肠球菌、金黄色葡萄球菌和白色念珠菌。DCD 蛋白在类似于汗液的条件下发挥最佳作用，还表现出蛋白水解活性，优先在 Arg 的 C 端侧裂解，在较小程度上裂解 Lys 残基。此外，DCD 蛋白可促进神经元存活并表现出磷酸酶活性，表明其在宿主防御机制中具有多方面的作用。此外，它可能具有与 IgG 结合的能力。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

P. pastoris

N-6*His

P81605-1 (Y20-L110)

117159  [NCBI]

YDPEAASAPGSGNPCHEASAAQKENAGEDPGLARQAPKPRKQRSSLLEKGLDGAKKAVGGLGKLGKDAVEDLESVGKGAVHDVKDVLDSVL

Approximately 17 kDa. The reducing (R) protein migrat es as 17 kDa in SDS-PAGE may be due to relative charge.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.