Draxin 蛋白, Human (HEK293, His)

The Draxin protein is critical in commissural development in the spinal cord and forebrain, serving as a chemical pulse guidance protein for commissural axons. It inhibits dorsal spinal cord neurite outgrowth and acts as a Wnt signaling antagonist by interacting with LRP6, inhibiting cytosolic β-catenin stabilization. Draxin Protein, Human (HEK293, His) is the recombinant human-derived Draxin protein, expressed by HEK293 , with C-His labeled tag.

Draxin 蛋白在脊髓和前脑连合的发育中起着至关重要的作用，充当连合轴突的化学脉冲引导蛋白。其功能延伸至抑制或排斥背侧脊髓的神经突生长。此外，Draxin 通过与 LRP6 相互作用抑制胞质 β-catenin (CTNNB1) 的稳定，从而充当 Wnt 信号通路的拮抗剂。这种多方面的功能强调了 Draxin 在指导轴突发育和调节神经发育过程中关键信号通路方面的重要性，强调了它在塑造神经系统复杂结构中的复杂参与。

Human

HEK293

C-His

Q8NBI3 (G26-V349)

374946  [NCBI]

GALAPGTPARNLPENHIDLPGPALWTPQASHHRRRGPGKKEWGPGLPSQAQDGAVVTATRQASRLPEAEGLLPEQSPAGLLQDKDLLLGLALPYPEKENRPPGWERTRKRSREHKRRRDRLRLHQGRALVRGPSSLMKKAELSEAQVLDAAMEESSTSLAPTMFFLTTFEAAPATEESLILPVTSLRPQQAQPRSDGEVMPTLDMALFDWTDYEDLKPDGWPSAKKKEKHRGKLSSDGNETSPAEGEPCDHHQDCLPGTCCDLREHLCTPHNRGLNNKCFDDCMCVEGLRCYAKFHRNRRVTRRKGRCVEPETANGDQGSFINV

Approximately 50-60 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.