EGFR 蛋白, Human (sf9, His-GST)

The EGFR protein is a receptor tyrosine kinase that can bind to a variety of ligands, such as EGF, TGFA, AREG, epigen, BTC, epiregulin, and HBEGF, to initiate signaling cascades that mediate cellular responses. This involves receptor dimerization, autophosphorylation and recruitment of adapter proteins such as GRB2, activating downstream pathways such as RAS-RAF-MEK-ERK, PI3-kinase-AKT, PLCgamma-PKC and STAT. EGFR Protein, Human (sf9, His-GST) is the recombinant human-derived EGFR protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of EGFR Protein, Human (sf9, His-GST) is 543 a.a., with molecular weight of ~89.1 kDa.

EGFR 蛋白是一种受体酪氨酸激酶，可结合 EGF 家族的配体，包括 EGF、TGFA/TGF-α、AREG、epigen/EPGN、BTC/betacellulin、epiregulin/EREG 和 HBEGF/肝素结合 EGF。这种相互作用启动级联反应，将细胞外信号转化为细胞反应，涉及受体同二聚化和/或异二聚化以及关键细胞质残基的自磷酸化。磷酸化受体招募 GRB2 等衔接蛋白，激活下游信号级联，包括 RAS-RAF-MEK-ERK、PI3 激酶-AKT、PLCgamma-PKC 和 STATs 模块。此外，EGFR 可能触发 NF-kappa-B 信号级联反应并直接磷酸化 RGS16 等蛋白质，激活其 GTPase 活性，从而可能将 EGF 受体信号传导与 G 蛋白偶联受体信号传导联系起来。此外，EGFR 磷酸化 MUC1，增强其与 SRC 和 CTNNB1/β-连环蛋白的相互作用。它通过与 CCDC88A/GIV 相互作用，正向调节细胞迁移，在配体刺激后将 EGFR 保留在细胞膜上，从而促进 EGFR 信号传导并触发细胞迁移。除了其典型功能外，EGFR 还有助于增强学习和记忆能力，并在哺乳动物疼痛信号传导中发挥作用，其中同工型 2 可能充当 EGF 作用的拮抗剂。

The specific activity was determined to be ≥4 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Human

Sf9 insect cells

N-His;N-GST

P00533-1 (M668-A1210)

1956  [NCBI]

MRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA

Approximately 89.1 kDa

Greater than 85% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.