EGFR 蛋白, Human (621a.a, Sf9, His)

EGFR Protein, Human (Sf9, His) is a glycosylated polypeptide chain with the C-termimal 6×His Tag produced in Sf9 insect cells. EGFR, belongs to a family of tyrosine kinase receptors, plays important roles in cell growth and differentiation.

表皮生长因子受体 (EGFR) 属于酪氨酸激酶受体家族，包括人 EGF 受体 (HER) 2、3 和 4，它们在细胞生长和分化中都发挥着重要作用。它们的主要配体是 EGF、肝素结合 EGF 和转化生长因子 α。配体结合后，EGFR 发生不对称二聚化，由“激活剂”和“受体”组成。EGFR 及其家族成员在多种癌症中失调。特别是，EGFR 在许多上皮实体瘤中过度表达。

Human

Sf9 insect cells

C-His

P00533-1 (L25-S645)

1956  [NCBI]

LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSHHHHHH

Approximately 80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Voldborg BR, et al. Epidermal growth factor receptor (EGFR) and EGFR mutations, function and possible role in clinical trials. Ann Oncol. 1997 Dec;8(12):1197-206.  [Content Brief]