EGFR 蛋白, Mouse (HEK293, His)

The EGFR protein is a receptor tyrosine kinase that binds EGF family ligands and activates multiple signaling cascades. Ligand binding induces receptor homo- or heterodimerization, leading to autophosphorylation and recruitment of adapter proteins (such as GRB2). EGFR Protein, Mouse (HEK293, His) is the recombinant mouse-derived EGFR protein, expressed by HEK293 , with C-10*His labeled tag.

EGFR 蛋白是一种受体酪氨酸激酶，可结合 EGF 家族的配体，例如 EGF、TGFA/TGF-α、AREG、epigen/EPGN、BTC/betacellulin、epiregulin/EREG 和 HBEGF/肝素结合 EGF，激活多种信号传导级联将细胞外信号转导为细胞反应。配体结合诱导受体同二聚和/或异二聚化，导致关键细胞质残基的自磷酸化。磷酸化受体招募 GRB2 等衔接蛋白，启动下游信号级联，包括 RAS-RAF-MEK-ERK、PI3 激酶-AKT、PLCgamma-PKC 和 STAT 模块。EGFR 还可能激活 NF-kappa-B 信号级联并直接磷酸化 RGS16 等蛋白质，从而调节 G 蛋白偶联受体信号传导。此外，EGFR 磷酸化 MUC1，增强其与 SRC 和 CTNNB1/β-连环蛋白的相互作用。值得注意的是，它通过与 CCDC88A/GIV 相互作用积极调节细胞迁移，促进 EGFR 信号传导并触发细胞迁移。EGFR 还在增强学习和记忆能力方面发挥作用，并有助于哺乳动物的疼痛信号传导，从而导致持久的超敏反应。

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2. Measured by its binding ability in a functional ELISA.
2.1. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind human EGF-Fc, The EC₅₀ of human EGF-Fc is 60-90 ng/mL.
2.2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind mouse EGF-Fc, The EC₅₀ of mouse EGF-Fc is 70-100 ng/mL.

Mouse

HEK293

C-10*His

Q01279 (L25-S647)

13649  [NCBI]

MRPSGTARTTLLVLLTALCAAGGALEEKKVCQGTSNRLTQLGTFEDHFLSLQRMYNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNALYENTYALAILSNYGTNRTGLRELPMRNLQEILIGAVRFSNNPILCNMDTIQWRDIVQNVFMSNMSMDLQSHPSSCPKCDPSCPNGSCWGGGEENCQKLTKIICAQQCSHRCRGRSPSDCCHNQCAAGCTGPRESDCLVCQKFQDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGPDYYEVEEDGIRKCKKCDGPCRKVCNGIGIGEFKDTLSINATNIKHFKYCTAISGDLHILPVAFKGDSFTRTPPLDPRELEILKTVKEITGFLLIQAWPDNWTDLHAFENLEIIRGRTKQHGQFSLAVVGLNITSLGLRSLKEISDGDVIISGNRNLCYANTINWKKLFGTPNQKTKIMNNRAEKDCKAVNHVCNPLCSSEGCWGPEPRDCVSCQNVSRGRECVEKCNILEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGIMGENNTLVWKYADANNVCHLCHANCTYGCAGPGLQGCEVWPSGPKIPS

Approximately 100 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.