ENSA 蛋白, Human (His)

ENSA protein is a key phosphatase inhibitor that precisely controls PP2A activity during mitosis by inhibiting PP2A. Phosphorylation of Ser-67 in mitosis promotes specific interaction with PPP2R2D, leading to inactivation of PP2A. ENSA Protein, Human (His) is the recombinant human-derived ENSA protein, expressed by E. coli , with N-6*His labeled tag.

ENSA 蛋白是一种关键磷酸酶抑制剂，在有丝分裂过程中对蛋白磷酸酶 2A (PP2A) 进行精确控制，特别是抑制其活性。有丝分裂期间 Ser-67 的磷酸化促进与 PPP2R2D (PR55-delta) 的特异性相互作用，导致 PP2A 失活。PP2A 的这种精心策划的失活对于在 M 期维持高细胞周期蛋白-B1-CDK1 活性至关重要，强调了 ENSA 在细胞周期进展中的调节作用。除了其有丝分裂功能外，ENSA 还通过与磺酰脲受体 (ABCC8) 相互作用，防止磺酰脲结合并减少 K(ATP) 通道电流，从而充当胰岛素分泌的刺激剂。值得注意的是，ENSA 与 PPP2R2D 和 ABCC8 的相互作用凸显了其在细胞周期控制和胰岛素分泌调节中的不同作用，展示了其在维持细胞稳态和功能完整性方面的重要性。此外，ENSA 与 SNCA 的相互作用在 Ser-109 磷酸化后被破坏，进一步强调了其对细胞过程的动态参与。

Human

E. coli

N-6*His

O43768-1 (M1-E121)

2029  [NCBI]

MSQKQEEENPAEETGEEKQDTQEKEGILPERAEEAKLKAKYPSLGQKPGGSDFLMKRLQKGQKYFDSGDYNMAKAKMKNKQLPSAGPDKNLVTGDHIPTPQDLPQRKSSLVTSKLAGGQVE

Approximately 17 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.