FGF-21 蛋白, Mouse

FGF-21 Protein, Mouse emerges as a metabolic hormone involved in the regulation of glucose, lipid, bile acid, and phosphate metabolism.

成纤维细胞生长因子 (FGF) 21 是 FGF 超家族的成员。它与 FGF19 和 FGF23 关系最密切，具有 30-35% 的氨基酸序列同源性。FGF19 亚家族包括 FGF19、FGF21 和 FGF23，所有三个 FGF19 亚家族成员最近均已成为参与葡萄糖、脂质、胆汁酸和磷酸盐代谢调节的代谢激素^[1]。FGF21 可提供持续的血糖和血脂控制、改善胰岛素抵抗、改善 β 细胞功能和质量，以及其他脂蛋白和心血管危险因素谱的有益变化^[2]。

1. The ED₅₀ is <0.5 μg/mL as measured by a cell proliferation assay using NIH-3T3 cells in the presence of 1.25 µg/mL mouse Klotho and 10.0 µg/mL heparin, corresponding to a specific activity of >2.0 × 10³ units/mg.
2. Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is ≤1.001 μg/mL in the presence of 1 μg/mL Recombinant Human Klotho beta, corresponding to a specific activity is ≥ 989.12 units/mg.

• 
  Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 1.001 μg/mL in the presence of 1 μg/mL Recombinant Human Klotho beta, corresponding to a specific activity is 989.12 units/mg.

Mouse

E. coli

Tag Free

Q9JJN1 (A29-S210)

56636  [NCBI]

AYPIPDSSPLLQFGGQVRQRYLYTDDDQDTEAHLEIREDGTVVGAAHRSPESLLELKALKPGVIQILGVKASRFLCQQPDGALYGSPHFDPEACSFRELLLEDGYNVYQSEAHGLPLRLPQKDSPNQDATSWGPVRFLPMPGLLHEPQDQAGFLPPEPPDVGSSDPLSMVEPLQGRSPSYAS

Approximately 20-23 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water or aqueous buffer containing 0.1% BSA.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Xu J, et al. Fibroblast growth factor 21 reverses hepatic steatosis, increases energy expenditure, and improves insulin sensitivity in diet-induced obese mice. Diabetes. 2009 Jan;58(1):250-9.  [Content Brief]

  [2]. Coskun T, et al. Fibroblast growth factor 21 corrects obesity in mice. Endocrinology. 2008 Dec;149(12):6018-27.  [Content Brief]