FOLR1 蛋白, Mouse (HEK293, His)

FOLR1 Protein, binding to folate and reduced folic acid derivatives, facilitates their delivery into cells. It maintains high affinity under neutral pH but undergoes a conformational change upon endocytosis, reducing affinity and releasing folates in slightly acidic pH. Crucial for embryonic development, cell proliferation, and renal folate reabsorption. FOLR1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FOLR1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of FOLR1 Protein, Mouse (HEK293, His) is 208 a.a., with molecular weight of 33-37 kDa.

FOLR1 蛋白与叶酸和还原叶酸衍生物结合，促进 5-甲基四氢叶酸和叶酸类似物输送到细胞中。在中性 pH 条件下，它对叶酸和叶酸类似物表现出高亲和力。内吞作用和暴露于弱酸性 pH 值时，会发生构象变化，导致其对叶酸的亲和力和随后的释放显着降低。这种蛋白质对于正常胚胎发育、细胞增殖和肾脏叶酸重吸收至关重要。

Mouse

HEK293

C-6*His

P35846 (T25-S232)

14275  [NCBI]

TRARTELLNVCMDAKHHKEKPGPEDNLHDQCSPWKTNSCCSTNTSQEAHKDISYLYRFNWNHCGTMTSECKRHFIQDTCLYECSPNLGPWIQQVDQSWRKERILDVPLCKEDCQQWWEDCQSSFTCKSNWHKGWNWSSGHNECPVGASCHPFTFYFPTSAALCEEIWSHSYKLSNYSRGSGRCIQMWFDPAQGNPNEEVARFYAEAMS

33-37 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.