Fractalkine/CX3CL1 蛋白, Human (S199N, CHO)

Fractalkine/CX3CL1 Protein, Human (S199N, CHO) is the member of CX3C chemokine family, associated with many cancers and promotes tumor progression.

Fractalkine 是迄今为止 CX3C 趋化因子家族中唯一的成员。它在许多细胞类型中表达，例如造血细胞、内皮细胞、上皮细胞、平滑肌细胞、树突状细胞、神经元和成纤维细胞。分形蛋白以两种形式存在，具有不同的细胞功能。可溶形式是表达 CX3CR1 受体的细胞的化学引诱剂。膜结合形式充当粘附分子，促进粘附和迁移^[1]。

The EC₅₀ is <1.5 µg/mL as measured on Ca²⁺ mobilization assay in CHO-K1/Gα15/hCX3CR1 cells (human Gα15 and hCX3CR1 stably expressed in CHO-K1 cells).

Human

CHO

Tag Free

P78423 (Q25-R339, S199N)

6376  [NCBI]

QHHGVTKCNITCSKMTSKIPVALLIHYQQNQASCGKRAIILETRQHRLFCADPKEQWVKDAMQHLDRQAAALTRNGGTFEKQIGEVKPRTTPAAGGMDESVVLEPEATGESSSLEPTPSSQEAQRALGTSPELPTGVTGSSGTRLPPTPKAQDGGPVGTELFRVPPVSTAATWQSSAPHQPGPSLWAEAKTSEAPSTQDPSTQASTASSPAPEENAPSEGQRVWGQGQSPRPENSLEREEMGPVPAHTDAFQDWGPGSMAHVSVVPVSSEGTPSREPVASGSWTPKAEEPIHATMDPQRLGVLITPVPDAQAATR

50-75 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Liu JF, et al. Fractalkine/CX3CL1 induced intercellular adhesion molecule-1-dependent tumor metastasis through the CX3CR1/PI3K/Akt/NF-κB pathway in human osteosarcoma. Oncotarget. 2016 Aug 12;8(33):54136-54148.  [Content Brief]