GDF-11/BMP-11 蛋白, Human (HEK293, solution)

The GDF-11/BMP-11 protein is a secreted signaling protein that globally regulates anterior/posterior axis patterning during development and plays a key role in mesoderm and neural tissue patterning. GDF-11/BMP-11 is critical for vertebral and orofacial development and signals through type 2 activin receptors (ACVR2A and ACVR2B) and type 1 activin receptors (ACVR1B, ACVR1C and TGFBR1), leading to SMAD2 and SMAD3 phosphorylation. GDF-11/BMP-11 Protein, Human (HEK293, solution) is the recombinant human-derived GDF-11/BMP-11 protein, expressed by HEK293 , with tag free. The total length of GDF-11/BMP-11 Protein, Human (HEK293, solution) is 109 a.a., with molecular weight of ~14.0 kDa.

GDF-11/BMP-11 是一种分泌信号蛋白，对发育过程中的前/后轴模式具有全局调节影响，并且在中胚层和神经组织模式中发挥关键作用。GDF-11/BMP-11 对于椎骨和口面部的正常发育至关重要，它通过 2 型激活素受体（ACVR2A 和 ACVR2B）和 1 型激活素受体（ACVR1B、ACVR1C 和 TGFBR1）传递信号，导致 SMAD2 和 TGFBR1 的磷酸化。SMAD3。该蛋白通过二硫键形成同二聚体，并直接与 ACVR2A、ACVR2B、ACVR1B、TGFBR1 和 ACVR1C 相互作用，后者以 ACVR2B 依赖性方式相互作用。此外，GDF-11/BMP-11 与 FST 同工型 2/FS-288 结合，突显其与信号通路中关键受体的复杂相互作用。

GDF-11/BMP-11 (5, 10, 20, and 40 ng/mL; 7 d) maintains the colony and cellular morphology of undifferentiated human embryonic stem cells (hESC), maintains POU5f1, NANOG, TRA-1-60, and SSEA4 expression, and displays increased SMAD2/3 phosphorylation in serum-free medium at 20 ng/mL concentration^[4].
GDF-11/BMP-11 (50-400 ng/mL; 2 d) induces browning of 3T3-L1 adipocytes via coordination of multiple signalling pathways, including mTORC1-COX2 and p38MAPK-PGC-1α as non-canonical pathways, as well as Smad1/5/8 as a canonical pathway^[5].

Human

HEK293

Tag Free

O95390 (N299-S407)

10220  [NCBI]

NLGLDCDEHSSESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYMFMQKYPHTHLVQQANPRGSAGPCCTPTKMSPINMLYFNDKQQIIYGKIPGMVVDRCGCS

Approximately 14.0-20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 50% glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.