GDF-15 蛋白, Mouse (HEK293, His-Flag)

The GDF-15 protein regulates food intake, energy balance, and body weight by binding to its receptor GFRAL.This activates GFRAL-expressing neurons in the brainstem, triggering "emergency circuits" in the central parabrachial nucleus and amygdala during stress.GDF-15 Protein, Mouse (HEK293, His-Flag) is the recombinant mouse-derived GDF-15 protein, expressed by HEK293 , with N-8*His, N-Flag labeled tag.

GDF-15 蛋白在调节食物摄入、能量消耗和体重以应对代谢和毒素引起的压力方面发挥着至关重要的作用。它通过与其受体 GFRAL 结合并激活位于脑干后区和孤束核的表达 GFRAL 的神经元来实现这一点。这种激活随后触发臂旁核和中央杏仁核内神经元的激活，这些神经元是“紧急回路”的一部分，负责在压力条件下形成进食反应。此外，GDF-15 蛋白可抑制肝细胞上的生长激素信号传导，并形成二硫键连接的同型二聚体。它还与 GFRAL 相互作用，作为配体通过与 RET 的相互作用促进 GDF15 内化和细胞信号传导。

Mouse

HEK293

N-8*His;N-Flag

Q9Z0J7 (S189-A303)

23886  [NCBI]

SAHAHPRDSCPLGPGRCCHLETVQATLEDLGWSDWVLSPRQLQLSMCVGECPHLYRSANTHAQIKARLHGLQPDKVPAPCCVPSSYTPVVLMHRTDSGVSLQTYDDLVARGCHCA

14-16 KDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.

Less than 1 EU/µg as determined by LAL test.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 4 mM HCl. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.