GFRA1/GDNFR-alpha-1 蛋白, Rat (HEK293, His)

GFRA1/GDNFR-α-1 protein is a receptor for GDNF and critically mediates GDNF-induced autophosphorylation and RET receptor activation.Presumably, two GDNFR-α-1 molecules form a complex with disulfide-linked GDNF, two RET molecules, and SORL1, suggesting the existence of a coordinated signaling mechanism.GFRA1/GDNFR-alpha-1 Protein, Rat (HEK293, His) is the recombinant rat-derived GFRA1/GDNFR-alpha-1 protein, expressed by HEK293 , with C-His, C-6*His labeled tag.

GFRA1/GDNFR-α-1 蛋白作为 GDNF 的受体，在介导 GDNF 诱导的自身磷酸化和 RET 受体的激活中发挥着至关重要的作用。有人提出，两个分子的 GDNFR-α-1 与二硫键连接的 GDNF 二聚体和两个分子的 RET 形成复合物，这表明信号转导的协调机制。GFRA1 与 RET 相互作用，促进与 GDNF 诱导的反应相关的复杂信号通路。此外，GFRA1 与 SORL1 相互作用，无论是单独的还是与 GDNF 形成复合物。这种相互作用导致 GFRA1 内化，而不导致其降解，表明 GFRA1 动态的调节方面。这些分子相互作用凸显了 GFRA1 在介导 GDNF 信号传导中的多方面作用，并强调了其在细胞过程中的重要性。

Measured in a cell proliferation assay using SH-SY5Y Human neuroblastoma cells. The ED₅₀ for this effect is ≤0.8977 μg/mL, corresponding to a specific activity is ≥1114 units/mg.

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  Measured in a cell proliferation assay using SH-SY5Y Human neuroblastoma cells.The ED₅₀ for this effect is 0.5294 μg/mL, corresponding to a specific activity is 1688.0486 units/mg.

Rat

HEK293

C-His

Q62997-1 (D25-L445)

25454  [NCBI]

DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLTSGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRAVPFISDVFQQVEHISKGNNCLDAAKACNLDDTCKKYRSAYITPCTTSMSNEVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEERERPNCLSLQDSCKTNYICRSRLADFFTNCQPESRSVSNCLKENYADCLLAYSGLIGTVMTPNYVDSSSLSVAPWCDCSNSGNDLEDCLKFLNFFKDNTCLKNAIQAFGNGSDVTMWQPAPPVQTTTATTTTAFRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGSTHLCLSDSDFGKDGLAGASSHITTKSMAAPPSCSL

Approximately 58-70 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.