1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. GLO2/Glyoxalase II 蛋白, Human (GST)

GLO2/Glyoxalase II 蛋白, Human (GST)

目录号: HY-P71690
产品使用指南

GLO2(即乙二醛酶 II 蛋白)作为硫酯酶,在催化 SD-乳酰谷胱甘肽的水解中发挥着至关重要的作用。这种活性会产生谷胱甘肽和 D-乳酸,强调了 GLO2 在细胞代谢中的重要性。该酶有助于调节谷胱甘肽水平和细胞途径内底物的转化。GLO2/Glyoxalase II 蛋白, Human (GST) 是重组的 GLO2/乙二醛酶 II 蛋白,由 E. coli 表达,带有 N-GST 标签。

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描述

GLO2, or Glyoxalase II Protein, serves as a thiolesterase, playing a crucial role in catalyzing the hydrolysis of S-D-lactoyl-glutathione. This activity generates glutathione and D-lactic acid, emphasizing GLO2's significance in cellular metabolism. The enzyme contributes to regulating glutathione levels and the conversion of substrates within cellular pathways. GLO2/Glyoxalase II Protein, Human (GST) is the recombinant human-derived GLO2/Glyoxalase II protein, expressed by E. coli , with N-GST labeled tag.

研究背景

GLO2,也称为乙二醛酶 II,作为硫醇酯酶发挥作用,在催化 SD-乳酰谷胱甘肽的水解中发挥关键作用。这种酶活性导致谷胱甘肽和 D-乳酸的形成。GLO2 在此过程中的具体作用强调了其在 SD-乳酰谷胱甘肽细胞代谢中的重要性,有助于谷胱甘肽水平的调节和细胞途径中涉及的底物的转化。

种属

Human

表达系统

E. coli

标签

N-GST

蛋白编号

Q16775-1 (K50-D308)

基因 ID
蛋白结构
N-term
GST
GLO2 (K50-D308)
Accession # Q16775-1
C-term
同用名
HAGH; GLO2; HAGH1Hydroxyacylglutathione hydrolase; mitochondrial; EC 3.1.2.6; Glyoxalase II; Glx II
氨基酸序列

KVEVLPALTDNYMYLVIDDETKEAAIVDPVQPQKVVDAARKHGVKLTTVLTTHHHWDHAGGNEKLVKLESGLKVYGGDDRIGALTHKITHLSTLQVGSLNVKCLATPCHTSGHICYFVSKPGGSEPPAVFTGDTLFVAGCGKFYEGTADEMCKALLEVLGRLPPDTRVYCGHEYTINNLKFARHVEPGNAAIREKLAWAKEKYSIGEPTVPSTLAEEFTYNPFMRVREKTVQQHAGETDPVTTMRAVRREKDQFKMPRD

分子量

Approximately 55.7 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

GLO2/Glyoxalase II Protein, Human (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GLO2/Glyoxalase II Protein, Human (GST)
目录号:
HY-P71690
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