1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. GRX1/Glutaredoxin 1 蛋白, Human (His)

GRX1/Glutaredoxin 1 蛋白, Human (His)

目录号: HY-P76366
COA 产品使用指南

GRX1(谷氧还蛋白 1)与 NADPH 和谷胱甘肽还原酶一起充当谷胱甘肽二硫化物氧化还原酶。它通过减少低分子量二硫化物和蛋白质发挥作用。GRX1/Glutaredoxin 1 蛋白, Human (His) 是重组的 GRX1/谷氧还蛋白 1 蛋白,由 E. coli 表达,带有 N-His 标签。GRX1/Glutaredoxin 1 蛋白, Human (His) 全长 106 个氨基酸,分子量约为 ~14 KDa。

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规格 价格 是否有货 数量
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5 μg ¥350 In-stock
10 μg ¥560 In-stock
50 μg ¥1300 In-stock
100 μg ¥1950 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

GRX1 (Glutaredoxin 1) acts as a glutathione-disulfide oxidoreductase with NADPH and glutathione reductase. It functions by reducing both low molecular weight disulfides and proteins. GRX1/Glutaredoxin 1 Protein, Human (His) is the recombinant human-derived GRX1/Glutaredoxin 1 protein, expressed by E. coli , with N-His labeled tag. The total length of GRX1/Glutaredoxin 1 Protein, Human (His) is 106 a.a., with molecular weight of ~14 KDa.

研究背景

当 NADPH 和谷胱甘肽还原酶存在时,GRX1(谷氧还蛋白 1)表现出谷胱甘肽二硫化物氧化还原酶活性。它的作用是减少低分子量二硫化物和蛋白质。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

P35754 (M1-Q106)

基因 ID
蛋白结构
N-term
His
GRX1 (M1-Q106)
Accession # P35754
C-term
同用名
Glutaredoxin-1; Thioltransferase-1; TTase-1; GLRX; GRX
氨基酸序列

MAQEFVNCKIQPGKVVVFIKPTCPYCRRAQEILSQLPIKQGLLEFVDITATNHTNEIQDYLQQLTGARTVPRVFIGKDCIGGCSDLVSLQQSGELLTRLKQIGALQ

分子量

Approximately 14 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

GRX1/Glutaredoxin 1 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GRX1/Glutaredoxin 1 Protein, Human (His)
目录号:
HY-P76366
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