IFN-gamma R1/CD119 蛋白, Mouse (HEK293, Fc)

IFN-gamma R1 (CD119), one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R1 forms the functional receptor with IFN-gamma R2. Upon binding with IFN-gamma, IFN-gamma R1 and IFN-gamma R2 oligomerize and transphosphorylate^[1]. Then, JAK1 and JAK2 are phosphorylated and activated, and STAT1 is recruited to the receptor complex. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54)^[2]. IFN-gamma R1/CD119 Protein, Mouse (HEK293, Fc) is a recombinant mouse IFN-gamma R1 (A26-D253) with C-terminal hFc tag, which is produced in HEK293 cells.

IFN-γ R1 (CD119) 是 IFN-γ 受体的亚基之一，是 IFN-γ 的受体。IFN-γ R1 组成型表达于几乎所有细胞的表面^[1]。
IFN-γ R1 可以与 IFN-γ R2 结合形成功能性受体。与 IFN-γ 结合后，IFNγR1 和 IFNγR2 寡聚并转磷酸化^[1]。然后，JAK1 和 JAK2 被磷酸化并激活，STAT1 被招募到受体复合物中。IFNγR1 的磷酸化为 STAT1 创建了一个对接位点，并导致 STAT1 磷酸化。磷酸化的 STAT1 易位到细胞核，在那里调节 IFN 反应基因（例如 CD54）的表达。IFN-γ R1 缺陷与 IFN-γ 介导的免疫反应以及感染易感性增加有关。IFN-gamma R1 信号通路对于激活癌细胞死亡和抑制癌症进展具有重要作用^[3]
小鼠 IFN-gamma R1 由胞外结构域 (A26-S254)、螺旋结构域 (I255) 组成-Y275)和胞质结构域(W276-S477)。IFNAR1的氨基酸序列在不同物种中有所不同。小鼠 IFN-γ R1 与人类具有 50% 的氨基酸序列同一性。IFN-gamma R1 在抗菌、抗病毒和抗肿瘤反应中发挥着关键作用^[2]。

IFN-gamma R1 (murine, -2 μg/mL, 4 h) suppresses AP(+)-exosome-mediated NO synthesis in RAW264.7 cells^[4].

Mouse

HEK293

C-hFc

P15261 (A26-D253)

15979  [NCBI]

70-75 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.

• [1]. Castro F, et al. Interferon-Gamma at the Crossroads of Tumor Immune Surveillance or Evasion. Front Immunol. 2018 May 4;9:847.  [Content Brief]

  [2]. van de Vosse E, et al. IFN-γR1 defects: Mutation update and description of the IFNGR1 variation database. Hum Mutat. 2017 Oct;38(10):1286-1296.  [Content Brief]

  [3]. Ding H, et al. Role of interferon-gamma (IFN-γ) and IFN-γ receptor 1/2 (IFNγR1/2) in regulation of immunity, infection, and cancer development: IFN-γ-dependent or independent pathway. Biomed Pharmacother. 2022 Nov;155:113683.  [Content Brief]

  [4]. Goto Y, et al. Contribution of the exosome-associated form of secreted endoplasmic reticulum aminopeptidase 1 to exosome-mediated macrophage activation. Biochim Biophys Acta Mol Cell Res. 2018 Jun;1865(6):874-888.  [Content Brief]