IL-12 蛋白, Human (HEK293)

IL-12 Protein, Human (HEK293) is a HEK293 cell derived heterodimeric, pro-inflammatory cytokine.

重组人 IL-12 (hIL-12) 来自稳定的 IL-12 转导的 HEK293 细胞的无血清上清液。Interleukin-12 是一种异二聚体促炎细胞因子，是细胞介导免疫的关键驱动因素。白细胞介素-12 (IL-12) 以前称为自然杀伤细胞刺激因子 (NKSF) 和细胞毒性淋巴细胞成熟因子 (CLMF)，是一种异二聚体细胞因子，由通过亚基间二硫桥连接的 p40 和 p35 亚基组成。在其众多多效性功能中，IL-12 已被证明可诱导：(i) IFNγ 产生；(ii) 活化的T细胞和NK细胞的增殖；(iii) T_H1 分化。这些功能是通过完整 IL-12 与其异二聚跨膜受体复合物结合介导的，该复合物由两条链 IL-12Rβ1 和 IL-12Rβ2^[1] 组成。

Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effects is < 2.0 ng/mL.

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  Measured in a cell proliferation assay using CTLL-2 cells. The ED₅₀ for this effect is 0.2209 ng/mL, corresponding to a specific activity is 4.53×10⁶ units/mg.

Human

HEK293

Tag Free

P29459 (R23-S219) & P29460 (I23-S328)

3592  [NCBI]&3593  [NCBI]

RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS&IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS

Approximately 32&44 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jayanthi S, et al. Efficient production and purification of recombinant human interleukin-12 (IL-12) overexpressed in mammalian cells without affinity tag. Protein Expr Purif. 2014 Oct;102:76-84.  [Content Brief]