IL-12 蛋白, Rat (HEK293, His)

IL-12 Protein forms the IL-12 cytokine and IL-35 cytokine. It regulates T-cell and natural killer-cell responses, stimulates interferon-gamma production, and promotes T-helper 1 cell differentiation. Its receptor, composed of IL12R1 and IL12R2 subunits, activates STAT4 and regulates gene expression. In the context of IL-35, IL-12 Protein maintains immune homeostasis and acts as an immune-suppressive cytokine. It signals through unconventional receptors and requires STAT1 and STAT4 transcription factors. It also interacts with NBR1 to promote IL-12 secretion. IL-12 Protein, Rat (HEK293, His) is a recombinant protein dimer complex containing rat-derived IL-12 protein, expressed by HEK293, with C-6*His labeled tag. IL-12 Protein, Rat (HEK293, His), has molecular weight of 25-35 & 40-50 kDa, respectively.

IL-12 蛋白能够与 IL12B 异二聚形成 IL-12 细胞因子，或与 EBI3/IL27B 异二聚形成 IL-35 细胞因子。它主要由 B 细胞、树突状细胞、巨噬细胞和粒细胞等专业抗原呈递细胞产生，在调节 T 细胞和自然杀伤细胞反应中发挥着至关重要的作用。IL-12 刺激干扰素 γ 的产生并促进 T 辅助细胞 1 细胞的分化，从而弥合先天抵抗和适应性免疫之间的差距。其生物效应通过由 IL12R1 和 IL12R2 亚基组成的受体介导，导致包括 TYK2 和 JAK2 在内的细胞底物磷酸化。这反过来又激活 STAT4 并促进细胞因子/生长因子反应基因的调节。在 IL-35 的背景下，IL-12 蛋白有助于维持肝脏微环境中的免疫稳态，并充当免疫抑制细胞因子。其信号传导通过由IL12RB2和gp130/IL6ST异二聚体或同二聚体组成的非常规受体发生，并且需要转录因子STAT1和STAT4的参与。此外，IL-12蛋白可以与IL12B形成二硫键连接的异二聚体，称为白细胞介素IL-12，以及与EBI3/IL27B形成非二硫键连接的异二聚体，称为白细胞介素IL-35。此外，它与 NBR1 相互作用，促进 IL-12 的分泌。

Rat

HEK293

C-6*His

Q9R103 (R23-S215)&Q9R278 (M23-S335)

84405  [NCBI]&64546  [NCBI]

MWELEKDVYVVEVDWRPDAPGETVTLTCDSPEEDDITWTSDQRRGVIGSGKTLTITVREFLDAGQYTCHRGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVHRNTDLKFNIKSSSSSPESRAVTCGRASLSAEKVTLNQRDYEKYSVACQEDVTCPTAEETLPIELVVEAQQQNKYENYSTSFFIRDIIKPDPPKNLQVKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKTKETEEECNQKGAFLVEKTSAEVQCKGANICVQAQDRYYNSSCSKWTCVPCRGRS&RVIPVSGPAKCLNQSQNLLKTTDDMVRTAREKLKHYSCTAGDIDHEDITRDKTSTLEACLPLELHKNESCLATKETSSIIRGSCLPPQKTSLMMTLCLGSIYEDLKMYQSEFQAINAALQSHNHQQITLDRNMLMAIDELMRSLNHSGETLHQKAPMGEADPYRVKMKLCILLHAFSTRVMTINRVMNYLSSS

25-35&40-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.