IL-18 蛋白, Mouse (His)

IL-18 protein is a pro-inflammatory cytokine that plays an important role in epithelial barrier repair and immune response regulation by polarizing T helper 1 (Th1) cells and natural killer (NK) cells. After binding to IL18R1 and IL18RAP receptors, IL-18 forms a signaling ternary complex, activates NF-κ-B, and induces inflammatory mediators. IL-18 Protein, Mouse (His) is the recombinant mouse-derived IL-18 protein, expressed by E. coli , with N-6*His labeled tag. The total length of IL-18 Protein, Mouse (His) is 157 a.a., with molecular weight of ~20.0 kDa.

IL-18 蛋白是一种促炎细胞因子，通过极化辅助 T 1 (Th1) 细胞和自然杀伤 (NK) 细胞，在上皮屏障修复和免疫反应调节中发挥着至关重要的作用。IL-18 与其受体 IL18R1 和 IL18RAP 结合后，形成信号三元复合物，激活 NF-κ-B，导致炎症介质的合成。它与 IL-12/interleukin-12 协同作用，诱导 Th1 细胞和 NK 细胞合成 IFNG。IL-18 还参与细胞焦亡下游的炎症转导，其成熟形式通过gasdermin-D (GSDMD) 孔特异性释放到细胞外空间。在质膜上，IL-18 与 IL18R1 和 IL18RAP 形成三元复合物，其中 IL18 首先与 IL18R1 结合形成低亲和力二元复合物，然后与 IL18RAP 相互作用形成高亲和力三元复合物，在细胞内发出信号。此外，IL-18 与货物受体 TMED10 相互作用，促进其从细胞质易位至内质网-高尔基中间室 (ERGIC) 进行分泌。

1.Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED₅₀ for this effect is 0.9227 ng/mL in the presence of 15 ng/mL TNF-alpha, corresponding to a specific activity is 1.08×10⁶ U/mg.
2.Measured by its ability to induce mouse IFN-gamma secretion by activated mouse T cells. The ED₅₀ for this effect is 0.1301 ng/mL, corresponding to a specific activity is 7.686×10⁶ U/mg.

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  Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED₅₀ for this effect is 0.9227 ng/ml in the presence of 15 ng/mL TNF-alpha, corresponding to a specific activity is 1.08×10⁶ U/mg.

Mouse

E. coli

N-6*His

P70380 (N36-S192)

16173  [NCBI]

NFGRLHCTTAVIRNINDQVLFVDKRQPVFEDMTDIDQSASEPQTRLIIYMYKDSEVRGLAVTLSVKDSKMSTLSCKNKIISFEEMDPPENIDDIQSDLIFFQKRVPGHNKMEFESSLYEGHFLACQKEDDAFKLILKKKDENGDKSVMFTLTNLHQS

Approximately 20.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 10% Sucrose, 3% Glycine, 100 mM NaCl, 0.1 mM EDTA, 0.05% Tween 80, pH 8.0 or 50 mM Tris-HCL, 200 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.