IL-18 蛋白, Rat

IL-18 Protein, Rat possesses immunoregulatory activities, including the stimulation of interferon (IFN)-γ production by T helper 1 (Th1) and natural killer cells, as well as other activities that promote inflammation.

白细胞介素 (IL)-18 是一种有效的免疫刺激剂，可通过增强辅助 T 1 (Th1) 细胞的活化来加重大鼠和小鼠 II 型胶原诱导的关节炎 (CIA) 的严重程度，从而增加促炎细胞因子和关节炎原性细胞因子的产生抗体。IL-18 具有免疫调节活性，包括刺激辅助 T 1 (Th1) 和自然杀伤细胞产生干扰素 (IFN)-g，以及促进炎症的其他活性^[1]。

1.The ED₅₀ is <2 μg/mL as measured by KG-1 cells, corresponding to a specific activity of >500 units/mg.
2.Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells. The ED50 for this effect is 0.055 μg/mL, corresponding to a specific activity is 1.818×10^4 U/mg.

Rat

E. coli

Tag Free

P97636-1 (H37-S194)

29197  [NCBI]

HFGRLHCTTAVIRSINDQVLFVDKRNPPVFEDMPDIDRTANESQTRLIIYMYKDSEVRGLAVTLSVKDGRMSTLSCKNKIISFEEMNPPENIDDIKSDLIFFQKRVPGHNKMEFESSLYEGHFLACQKEDDAFKLVLKRKDENGDKSVMFTLTNLHQS

Approximately 18.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 50 mM NaCl, pH 8.0 or PBS, pH 7.4, 8% trehalose.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Ye XJ, et al. The effects of interleukin-18 on rat articular chondrocytes: a study of mRNA expression and protein synthesis of proinflammatory substances. Clin Exp Immunol. 2007 Sep;149(3):553-60.  [Content Brief]