IL-1RA/IL-1RN 蛋白, Human

IL-1RA/IL-1RN Protein, Human is a member of the IL-1 family that binds to IL-1 receptors.

人白细胞介素 1 受体拮抗剂蛋白 (IL-1ra) 的产生受到许多物质的刺激，包括粘附的 IgG、其他细胞因子以及细菌或病毒成分。内源性 IL-1Ra 在许多疾病实验动物模型以及人类自身免疫和慢性炎症疾病中产生。使用重组人 IL-1Ra 治疗人类疾病对败血症综合征没有益处^[1]。IL-1 受体拮抗剂 (IL-1ra) 提供了一种体内阻断 IL-1 活性的工具，使人们能够详细了解这种细胞因子在炎症反应中的作用^[2]。

Measured by its ability to inhibit IL-1 alpha -dependent proliferation in CTLL-2. The ED₅₀ for this effect is 0.6883-1.145 ng /mL in the presence of 50 pg/mL of recombinant porcine IL-1 alpha, corresponding to a specific activity is 8.73×10⁵-1.45×10⁶ units/mg.

• 
  Measured by its ability to inhibit IL-1 alpha -dependent proliferation in CTLL-2. The ED₅₀ for this effect is 0.6883 ng /mL in the presence of 50 pg/mL of recombinant porcine IL-1 alpha, corresponding to a specific activity is1.45×10⁶ units/mg.

Human

E. coli

Tag Free

P18510-1 (R26-E177)

3557  [NCBI]

RPSGRKSSKMQAFRIWDVNQKTFYLRNNQLVAGYLQGPNVNLEEKIDVVPIEPHALFLGIHGGKMCLSCVKSGDETRLQLEAVNITDLSENRKQDKRFAFIRSDSGPTTSFESAACPGWFLCTAMEADQPVSLTNMPDEGVMVTKFYFQEDE

15-19 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 8% Trehalose, 4% Mannitol, 50 mM NaCl, 0.05% Tween 80, pH 7.5 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Arend WP, et al. Interleukin-1 receptor antagonist: role in biology. Annu Rev Immunol. 1998;16:27-55.  [Content Brief]

  [2]. Nikolic-Paterson DJ, et al. Interleukin-1 receptor antagonism. Semin Nephrol. 1996 Nov;16(6):583-90.  [Content Brief]

  [3]. Arend WP, et al. Interleukin 1 receptor antagonist. A new member of the interleukin 1 family. J Clin Invest. 1991 Nov;88(5):1445-51.  [Content Brief]