IL-2 蛋白, Rat (145a.a, HEK293, C-His)

IL-2 Protein, produced by activated T-cells, NK cells, and helper T-cells, is crucial for immune response and tolerance. It binds to receptor complexes, activating downstream signaling pathways. IL-2 Protein acts as a growth factor, enhances NK-cell activity, promotes B-cell proliferation, and regulates regulatory T-cells. It also participates in differentiating effector T-cell subsets and memory CD8-positive T-cells. IL-2 Protein, Rat (145a.a, HEK293, C-His) is the recombinant rat-derived IL-2 protein, expressed by HEK293 , with C-10*His labeled tag.

IL-2 是一种细胞因子，主要由活化的 CD4 阳性辅助 T 细胞产生，少量由活化的 CD8 阳性 T 细胞和自然杀伤 (NK) 细胞产生，在免疫反应和耐受中发挥着关键作用。它与由高亲和力三聚体 IL-2R（IL2RA/CD25、IL2RB/CD122 和 IL2RG/CD132）或低亲和力二聚体 IL-2R（IL2RB 和 IL2RG）组成的受体复合物结合。这种相互作用触发 IL-2R 亚基的寡聚化和构象变化，启动以 JAK1 和 JAK3 磷酸化为起始的下游信号传导。因此，JAK1 和 JAK3 磷酸化受体，为底物（包括 STAT5）磷酸化创建对接位点，激活 STAT、磷酸肌醇 3-激酶/PI3K 和丝裂原激活蛋白激酶/MAPK 等途径。IL-2 作为 T 细胞生长因子发挥作用，增强 NK 细胞的溶细胞活性，并促进活化 B 细胞的强劲增殖，从而增加免疫球蛋白的产生。至关重要的是，IL-2 通过控制调节性 T 细胞的存活和增殖，在调节适应性免疫系统中发挥着关键作用，这对于维持免疫耐受至关重要。此外，它还参与效应 T 细胞亚群的分化和稳态，包括 Th1、Th2、Th17 以及记忆 CD8 阳性 T 细胞。

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED₅₀ this effect is 0.1884 ng/mL, corresponding to a specific activity is 5.307×10⁶ units/mg.

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  Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED₅₀ this effect is 0.1884 ng/mL, corresponding to a specific activity is 5.307×10⁶ units/mg.

Rat

HEK293

C-10*His

P17108 (A11-Q155)

116562  [NCBI]

ALTLVLLVNSAPTSSPAKETQQHLEQLLLDLQVLLRGIDNYKNLKLPMMLTFKFYLPKQATELKHLQCLENELGALQRVLDLTQSKSFHLEDAGNFISNIRVTVVKLKGSENKFECQFDDEPATVVEFLRRWIAICQSIISTMTQ

Approximately 18 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.