IL-3 蛋白, Human (His)

IL-3 protein is mainly secreted by T lymphocytes, mast cells and osteoblasts and is critical for the generation and differentiation of hematopoietic progenitor cells. It stimulates mature basophils, eosinophils and monocytes, promoting functional activation. IL-3 Protein, Human (His) is the recombinant human-derived IL-3 protein, expressed by E. coli , with N-6*His labeled tag.

细胞因子 IL-3 主要由活化的 T 淋巴细胞、肥大细胞和成骨细胞分泌，在控制造血祖细胞向谱系限制细胞的产生和分化中发挥着至关重要的作用。此外，IL-3 还可刺激成熟的嗜碱性粒细胞、嗜酸性粒细胞和单核细胞，促进其功能激活。除了造血功能外，IL-3 还有助于神经细胞增殖和存活。此外，它还通过防止 NF-κ-B 核转位和激活来抑制破骨细胞分化，从而参与骨稳态。从机制上讲，IL-3通过由IL3RA亚基和信号转导亚基IL3RB组成的受体发挥其生物学作用。刺激该受体会导致 JAK2 激酶活性快速激活，启动 STAT5 介导的转录程序。另外，IL-3 通过激活 PI3K/AKT 和 ERK 介导的途径，有助于非造血系统中氧化应激下的细胞存活。该细胞因子还与 IL3RA 相互作用以调节其多种生理效应。

1.The cell proliferation assay using TF-1 human erythroleukemic cells has an ED₅₀ value of 0.05-0.3 ng/mL.
2.Loaded Human IL-3RA-Fc on Protein A Biosensor, can bind Human IL-3 with an affinity constant of 3.89 μM as determined in BLI assay.
3.Loaded Human IL-3RA-Fc-Avi on Protein A Biosensor, can bind Human IL-3 with an affinity constant of 3.74 μM as determined in BLI assay.

Human

E. coli

N-6*His

P08700 (A20-F152)

3562  [NCBI]

APMTQTTPLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF

Approximately 13-17 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mMPB, 5%Sucrose, 0.05%Tween80, pH 6.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.