IL-36 beta/IL-1F8 蛋白, Human (His)

IL-36 beta (IL-1F8), a subform of IL-36 family, belongs to IL-1 superfamily. IL-36 beta mediates inflammatory response. L-36 beta binds to IL-36R and recruits the co-receptor IL-1RacP, and thereby activating NF-κB and MAPK signaling pathways, but the activation requires N-terminal cleavage by neutrophil granule-derived proteases^[1][2]. IL-36 beta/IL-1F8 Protein, Human (His) is a recombinant human IL-36 beta with N terminal His tag, which is produced in E. coli.

IL-36β（IL-1F8）是IL-36家族的一个亚型，属于IL-1超家族。IL-36 beta 在单核细胞、T/B 淋巴细胞、骨髓、扁桃体、心脏、肺、睾丸、结肠、神经元细胞、神经胶质细胞中表达^[3]。
IL-36β 的氨基酸序列在不同物种中有所不同。人 IL-36 β 与小鼠的氨基酸序列同一性<40%。
L-36 beta 与 IL-36R 结合并招募辅助受体 IL-1RAcP。因此，异二聚体信号复合物使 2 条受体链的 Toll/IL-1R (TIR) 结构域非常接近，从而激活 NF-κB 和 MAPK 信号通路^[1]。但激活需要中性粒细胞颗粒衍生的蛋白酶（例如组织蛋白酶 G、弹性蛋白酶和蛋白酶-3^[2]）在 Arg5 的 N 端进行切割。IL-36β在人骨髓单核细胞和DC细胞的细胞成熟中发挥作用。IL-36β 与炎症性肠病 (IBD) 的发生有关。IBD 患者的血清 IL36β 水平通常较高^[4]。
IL-36β 是一种促炎因子。IL-36 beta 通过激活 NF-κB 和 MAPK 信号通路介导炎症反应^[2]。

IL-36 beta (human) (1 μg/mL, 24 h) induces the gene expression of themselves and other family members in keratinocytes^[5].
IL-36 beta (human) (1-5 ng/mL) increases IL-36R mRNA level in human blood lymphocytes^[6].
IL-36 beta (human) (1 ng/mL, 48 h) induces early proliferation of IL-36R expressing CD4+ T helper cells^[6].

Human

E. coli

N-His

Q9NZH7-2 (K5-E157)

27177  [NCBI]

Approximately 20 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.

• [1]. Bassoy EY, et al. Regulation and function of interleukin-36 cytokines. Immunol Rev. 2018 Jan;281(1):169-178.  [Content Brief]

  [2]. Zhou L,et al. Interleukin-36: Structure, Signaling and Function. Adv Exp Med Biol. 2021;21:191-210.  [Content Brief]

  [3]. Gresnigt MS, et al. Biology of IL-36 cytokines and their role in disease. Semin Immunol. 2013 Dec 15;25(6):458-65.  [Content Brief]

  [4]. Zhu J, et al. Interleukin-36β exacerbates DSS-induce acute colitis via inhibiting Foxp3+ regulatory T cell response and increasing Th2 cell response. Int Immunopharmacol. 2022 Jul;108:108762.  [Content Brief]

  [5]. Carrier Y, et al. Inter-regulation of Th17 cytokines and the IL-36 cytokines in vitro and in vivo: implications in psoriasis pathogenesis. J Invest Dermatol. 2011 Dec;131(12):2428-37.  [Content Brief]

  [6]. Penha R, et al. IL-36 receptor is expressed by human blood and intestinal T lymphocytes and is dose-dependently activated via IL-36β and induces CD4+ lymphocyte proliferation. Cytokine. 2016 Sep;85:18-25.  [Content Brief]