IL-7 蛋白, Human

IL-7 Protein, Human, a hematopoietic cytokine, promotes T-cell recovery after allogeneic stem cell transplantation.

重组人白细胞介素 7 (r-hIL-7) 在 T 细胞发育和存活中发挥核心作用，并增强同种异体造血干细胞移植 (allo-HSCT) 小鼠模型的免疫恢复。重组人白细胞介素 7 诱导 CD4⁺ 和 CD8⁺ T 细胞倍增。IL-7 的主要作用是效应记忆 T 细胞的扩增。r-hIL-7 可以增强 T 细胞耗尽的异基因 HSCT 后的免疫恢复，而不会引起明显的 GVHD 或其他严重毒性^[1]。

1.The ED₅₀ is <0.5 ng/mL as measured by murine 2E8 cells, corresponding to a specific activity of >2.0 × 10⁶ units/mg.
2.Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED₅₀ for this effect is 0.02-0.08 ng/mL.
3.Measured in a cell proliferation assay using Jurkat cells. The ED₅₀ for this effect is 0.4102 ng/mL, corresponding to a specific activity is 2.438×10⁶ units/mg.

• 
  Measured in a cell proliferation assay using Jurkat cells. The ED₅₀ for this effect is 0.4102 ng/mL, corresponding to a specific activity is 2.438×10⁶ units/mg.

Human

E. coli

Tag Free

P13232-1 (D26-H177)

3574  [NCBI]

DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH

Approximately 17-20 kD.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Perales MA, et al. Recombinant human interleukin-7 (CYT107) promotes T-cell recovery after allogeneic stem cell transplantation. Blood. 2012 Dec 6;120(24):4882-91.  [Content Brief]