IL-7 蛋白, Human (HEK293, His)

IL-7 protein is an important hematopoietic cytokine that is essential for the development, expansion and survival of T cells and B cells, regulating mature lymphocyte populations and maintaining lymphatic homeostasis. IL-7 interacts with IL7RA and CSF2RG subunits, activates kinases, including JAK1 or JAK3, and initiates signaling cascades, such as the PI3K/Akt/mTOR or JAK-STAT5 pathway. IL-7 Protein, Human (HEK293, His) is the recombinant human-derived IL-7 protein, expressed by HEK293 , with C-6*His labeled tag.

IL-7蛋白作为重要的造血细胞因子，在幼稚T细胞、记忆T细胞以及B细胞的发育、扩增和存活中发挥着不可或缺的作用，从而调节成熟淋巴细胞的数量并维持淋巴稳态。其生物学效应是通过由IL7RA亚基和细胞因子受体共同亚基γ/CSF2RG组成的受体来执行的。与受体结合后，IL-7 会激活各种激酶，包括 JAK1 或 JAK3，具体取决于细胞类型。这种激活导致信号通过多个下游途径传播，例如 PI3K/Akt/mTOR 或 JAK-STAT5 途径。IL-7 与 IL7R 和 CSF2RG 的相互作用凸显了其在协调对免疫细胞发育和功能至关重要的多种信号级联中的关键作用。

1.The cell proliferation assay using PHA-activated human peripheral blood lymphocytes has an ED₅₀ value of 50-300 pg/mL.
2. Mouse IL-7RA-Fc can bind Human IL-7-His with an affinity constant of 29.0 nM as determined in BLI assay.
3. Measured in a cell proliferation assay using HT-2 mouse T lymphocyte cells. The ED₅₀ this effect is ≤1.822 ng/mL, corresponding to a specific activity of ≥5.489×10⁵ units/mg.

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  1.The cell proliferation assay using PHA-activated human peripheral blood lymphocytes has an ED₅₀ value of 50-300 pg/mL. Mouse IL-7RA-Fc can bind Human IL-7-His with an affinity constant of 29.0 nM. 2. Measured in a cell proliferation assay using HT-2 mouse T lymphocyte cells. The ED₅₀ this effect is 1.822 ng/mL, corresponding to a specific activity is 5.489×10⁵ units/mg.

Human

HEK293

C-6*His

P13232-1 (D26-H177)

3574  [NCBI]

DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH

Approximately 19-32 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.