Kallikrein-7 蛋白, Human (HEK293, His, solution)

Kallikrein-7 Protein (KLK7), a member of the tissue kallikrein family, is a first protease target of vaspin inhibited by classical serpin mechanism with high specificity in vitro. KLK7 cleaves human insulin in the A- and B-chain. KLK7 a serine protease with chymotrypsin-like activity which was involved in the regulated desquamation of terminally differentiated keratinocytes. KLK7 mediates the disruption of corneodesmosomes, the cell–cell adhesion junctions of corneocyites, by hydrolyzing the two mayor cadherins (corneodesmosin and desmocollin 1) in the extracellular region of these junctions. KLK7 overexpression and/or increased activity result in over-desquamation^[1][2][3]. Kallikrein-7 Protein, Human (HEK293, His, solution) is the recombinant human-derived Kallikrein-7 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Kallikrein-7 Protein, Human (HEK293, His, solution) is 230 a.a., with molecular weight of ~30.0 kDa.

全长成熟KLK7的分子量预计为~25 kDa，并且KLK7在246NDT处有一个预测的糖基化位点，该位点远离KLK7催化三联体和底物结合口袋^[1]。
KLK7 是组织激肽释放酶家族的成员，该家族包含 15 种人类胰凝乳蛋白酶或胰蛋白酶样丝氨酸蛋白酶。KLK7 主要在皮肤中表达，参与脱皮过程。KLK7 活性异常是银屑病、酒渣鼻和 Netherton 综合征等炎症性皮肤病的主要潜在致病机制^[51]。
KLK7 最初被定性为一种酶，与复层鳞状上皮角质层中细胞间粘附结构的降解有关，随后发生皮肤脱屑。它催化皮肤最外层桥粒的降解，并允许皮肤表面发生细胞脱落。据报道，肿瘤细胞中 KLK7 的过度表达可显着增强颅内恶性肿瘤和卵巢癌细胞的侵袭潜力。因此，KLK7可以促进口腔鳞状细胞癌（OSCC）组织中细胞外基质的降解，促进肿瘤细胞局部侵袭并促进区域淋巴结转移^[2]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

AAH32005 (E23-H252)

5650  [NCBI]

EEAQGDKIIDGAPCARGSHPWQVALLSGNQLHCGGVLVNERWVLTAAHCKMNEYTVHLGSDTLGDRRAQRIKASKSFRHPGYSTQTHVNDLMLVKLNSQARLSSMVKKVRLPSRCEPPGTTCTVSGWGTTTSPDVTFPSDLMCVDVKLISPQDCTKVYKDLLENSMLCAGIPDSKKNACNGDSGGPLVCRGTLQGLVSWGTFPCGQPNDPGVYTQVCKFTKWINDTMKKH

Approximately 28-32 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.