L-selectin/CD62L 蛋白, Human (HEK293, His)

The L-selectin/CD62L protein is a calcium-dependent lectin that promotes cell adhesion by binding to glycoproteins on neighboring cells. L-selectin/CD62L Protein, Human (HEK293, His) is the recombinant human-derived L-selectin/CD62L protein, expressed by HEK293 , with C-His labeled tag. The total length of L-selectin/CD62L Protein, Human (HEK293, His) is 294 a.a., with molecular weight of 50-75 kDa.

L-选择素，也称为 CD62L，是一种钙依赖性凝集素，通过与邻近细胞上的糖蛋白结合，在介导细胞粘附方面发挥着至关重要的作用。从功能上讲，它促进淋巴细胞粘附到外周淋巴结高内皮微静脉内的内皮细胞，促进内皮组织中白细胞的初始束缚和滚动。L-选择素与 SELPLG/PSGL1 和 PODXL2 的相互作用对于白细胞的招募和滚动至关重要。这种相互作用特别依赖于 SELPLG 和 PODXL2 的唾液酸路易斯 X 聚糖修饰，以及 SELPLG 的酪氨酸硫酸化修饰。值得注意的是，SELPLG 'Tyr-51' 上的硫酸化成为有效 L-选择蛋白结合的关键因素。L-选择素的多方面功能强调了其在协调细胞粘附过程和免疫细胞招募中的重要性。

Measured by the ability of the immobilized protein to support the adhesion of THP-1 human prostate cancer cells. The ED₅₀ for this effect is 2.231 μg/mL, corresponding to a specific activity is 448.230 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of THP-1 human prostate cancer cells.  The ED₅₀ for this effect is 2.231 μg/mL, corresponding to a specific activity is 448.230 units/mg.

Human

HEK293

C-His

P14151 (W39-N332)

6402  [NCBI]

WTYHYSEKPMNWQRARRFCRDNYTDLVAIQNKAEIEYLEKTLPFSRSYYWIGIRKIGGIWTWVGTNKSLTEEAENWGDGEPNNKKNKEDCVEIYIKRNKDAGKWNDDACHKLKAALCYTASCQPWSCSGHGECVEIINNYTCNCDVGYYGPQCQFVIQCEPLEAPELGTMDCTHPLGNFSFSSQCAFSCSEGTNLTGIEETTCGPFGNWSSPEPTCQVIQCEPLSAPDLGIMNCSHPLASFSFTSACTFICSEGTELIGKKKTICESSGIWSNPSPICQKLDKSFSMIKEGDYN

50-75 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.