L-selectin/CD62L 蛋白, Mouse (294a.a, HEK293, C-Fc-His)

L-selectin, a calcium-dependent lectin, binds to glycoproteins on adjacent cells, facilitating lymphocyte attachment to endothelial cells in peripheral lymph nodes.This interaction is essential for leukocyte rolling along the endothelium and requires L-selectin's binding to SELPLG/PSGL1 and PODXL2, dependent on glycan and sulfation modifications.Sulfation of 'Tyr-51' on SELPLG is particularly important for L-selectin binding.L-selectin/CD62L Protein, Mouse (HEK293, C-Fc-His) is the recombinant mouse-derived L-selectin/CD62L protein, expressed by HEK293 , with C-6*His, C-Fc labeled tag.

L-选择素是一种钙依赖性凝集素，通过与相邻细胞上的糖蛋白结合，在细胞粘附中发挥着至关重要的作用。具体来说，它促进淋巴细胞附着到周围淋巴结内高内皮微静脉中的内皮细胞，促进白细胞沿着内皮的初始束缚和滚动。该过程需要 L-选择素与 SELPLG/PSGL1 和 PODXL2 相互作用，这依赖于 SELPLG 和 PODXL2 的唾液酸路易斯 X 聚糖修饰，以及 SELPLG 的酪氨酸硫酸化修饰。值得注意的是，SELPLG 上“Tyr-51”的硫酸化对于 L-选择素的结合特别重要。

Mouse

HEK293

C-hFc;C-6*His

P18337 (W39-N332)

20343  [NCBI]

WTYHYSEKPMNWENARKFCKQNYTDLVAIQNKREIEYLENTLPKSPYYYWIGIRKIGKMWTWVGTNKTLTKEAENWGAGEPNNKKSKEDCVEIYIKRERDSGKWNDDACHKRKAALCYTASCQPGSCNGRGECVETINNHTCICDAGYYGPQCQYVVQCEPLEAPELGTMDCIHPLGNFSFQSKCAFNCSEGRELLGTAETQCGASGNWSSPEPICQVVQCEPLEAPELGTMDCIHPLGNFSFQSKCAFNCSEGRELLGTAETQCGASGNWSSPEPICQETNRSFSKIKEGDYN

90-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.