LILRB1/CD85j/ILT2 蛋白, Human (435a.a, HEK293, His)

The LILRB1/CD85j/ILT2 protein is a receptor for class I MHC antigens and recognizes multiple HLA alleles and H301/UL18 (human cytomegalovirus MHC homolog). Ligand binding induces inhibitory signals that downregulate immune responses. LILRB1/CD85j/ILT2 Protein, Human (435a.a, HEK293, His) is the recombinant human-derived LILRB1/CD85j/ILT2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of LILRB1/CD85j/ILT2 Protein, Human (435a.a, HEK293, His) is 435 a.a., with molecular weight of 65-90 kDa.

LILRB1/CD85j/ILT2 蛋白作为 I 类 MHC 抗原的受体，可广泛识别 HLA-A、HLA-B、HLA-C、HLA-G 和 HLA-F 等位基因。此外，它还充当 H301/UL18（人类巨细胞病毒 I 类 MHC 同源物）的受体。配体结合诱导抑制信号，导致免疫反应下调。LILRB1 与自然杀伤细胞或 T 细胞上的 I 类 MHC 分子的结合可保护靶细胞免于裂解，而与 HLA-B 或 HLA-E 的相互作用会抑制 FCER1A 信号传导和血清素释放。此外，LILRB1 抑制 FCGR1A 介导的细胞反应，包括蛋白质磷酸化和细胞内钙离子的动员。它识别与 B2M/β-2 微球蛋白和九聚体自肽复合的 HLA-G，触发蜕膜 NK 细胞分泌生长促进因子。此外，它还能将 B 细胞重新编程为免疫抑制表型。LILRB1 磷酸化时结合 PTPN6，并与来自人巨细胞病毒的 FCER1A、FCGR1A 和 UL18 蛋白相互作用。它还与肽结合的 HLA-G-B2M 和 HLA-F-B2M 复合物相互作用，突出了其在免疫调节和病毒识别中的多种作用。

Human

HEK293

C-6*His

ADJ55949.1 (G24-H458)

10859  [NCBI]

GHLPKPTLWAEPGSVITQGSPVTLRCQGGQETQEYRLYREKKTAPWITRIPQELVKKGQFPIPSITWEHAGRYRCYYGSDTAGRSESSDPLELVVTGAYIKPTLSAQPSPVVNSGGNVTLQCDSQVAFDGFILCKEGEDEHPQCLNSQPHARGSSRAIFSVGPVSPSRRWWYRCYAYDSNSPYEWSLPSDLLELLVLGVSKKPSLSVQPGPIVAPEETLTLQCGSDAGYNRFVLYKDGERDFLQLAGAQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSEWSAPSDPLDILIAGQFYDRVSLSVQPGPTVASGENVTLLCQSQGWMQTFLLTKEGAADDPWRLRSTYQSQKYQAEFPMGPVTSAHAGTYRCYGSQSSKPYLLTHPSDPLELVVSGPSGGPSSPTTGPTSTSGPEDQPLTPTGSDPQSGLGRH

65-90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.